CD105 MultiSort Kit (PE), mouse

CD105 MultiSort Kit (PE), mouse

The CD105 MultiSort Kit (PE), mouse has been developed for the isolation of CD105
cells or cell subsets, including
  • vascular endothelial cells from mouse tissue or cell cultures
  • long-term repopulating hematopoietic stem cells (LTR-HSCs) from mouse bone marrow when used in combination with the Anti-Sca-1 MicroBead Kit (FITC).

Background information

CD105, also known as Endoglin, is abundantly expressed on angiogenic endothelial cells. In mouse bone marrow, CD105 is also expressed on a subpopulation of Sca-1
hematopoietic stem cells that displays long-term repopulating activity in lethally irradiated mice (LTR-HSCs)

Detailed separation procedure

In the first step, CD105
cells are positively selected after labeling with the CD105 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD105 antibody. The selected CD105
cells can be magnetically labeled and sorted according to a second antigen. The kits contains CD105 MultiSort MicroBeads, MultiSort Release Reagent, and the MultiSort Stop Reagent.

Downstream applications

Isolated CD105
cells can be transplanted into lethally irradiated mice to evaluate the long-term engraftment potential of this cell population.


MS, LS, or autoMACS
  • Selected references

    1. Chen, C. Z. et al. (2002) Identification of endoglin as a functional marker that defines long-term repopulating hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A. 99: 15468-15473
    2. Chen, C. Z. et al. (2003) The endoglin(positive) sca-1(positive) rhodamine(low) phenotype defines a near-homogeneous population of long-term repopulating hematopoietic stem cells. Immunity 19: 525-533
  • Special protocols

Product options: 1
for 1×
total cells
GBP 550.00

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