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Myeloid-derived suppressor cells were isolated from a spleen of a BALB/c mouse using the Myeloid-Derived Suppressor Cell Isolation Kit, two LS Columns, two MS Columns, and an appropriate MACS Separator. Cells were fluorescently stained with CD11b-APC and Anti-Gr-1-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Spleen cells before separation
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