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Mouse embryonic fibroblasts (mEF) were depleted from a co-culture with mouse embryonic stem (mES) cells using Feeder Removal MicroBeads, an LS Column, and a MidiMACS™ Separator. Flow cytometric analysis with CD15 (SSEA-1)-APC and Anti-Feeder-PE on the MACSQuant® Analyzer indicates that a starting population of 10% mEF was completely removed. The purity of the enriched mES cells was about 99.9%.
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