Plasmacytoid Dendritic Cell Isolation Kit, mouse

Plasmacytoid Dendritic Cell Isolation Kit, mouse

The Plasmacytoid Dendritic Cell Isolation Kit is designed for the isolation of untouched mouse plasmacytoid dendritic cells (PDCs) from single-cell suspensions of lymphoid organs.

Background information

Freshly isolated PDCs express low levels of MHC class II and costimulatory molecules and are poor stimulators of T helper cells. Upon stimulation with bacterial DNA containing particular unmethylated CpG motifs (CpG-DNA) or upon viral challenge, they produce large amounts of type I IFN and acquire antigen-presenting capacity.
1,2
PDCs can be directly identified in lymphoid and non-lymphoid organs by the specific expression of mPDCA-1 (murine plasmacytoid dendritic cell antigen-1).
3
mPDCA-1 has been identified as bone marrow stromal antigen 2 (BST2).
4

Detailed separation procedure

Using the Plasmacytoid Dendritic Cell Isolation Kit, non-PDCs are labeled with a cocktail of biotin-conjugated monoclonal antibodies and Anti-Biotin MicroBeads and depleted by retaining them on a MACS
®
Column in the magnetic field of a MACS Separator, while the highly enriched unlabeled PDCs pass through the column.

Downstream applications

PDCs isolated by using the Plasmacytoid Dendritic Cell Isolation Kit can be used to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T helper cell polarization by PDCs.

Columns

MS, LS, or autoMACS
®
Columns.
  • Selected references

    1. Blasius, A. L. et al. (2006) Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in the naive mouse, but a promiscuous cell surface antigen following IFN stimulation. J. Immunol. 177: 3260-3265
    2. Nakano, H. et al. (2001)
      CD11c
      +
      B220
      +
      Gr-1
      +
      cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells.
      J. Exp. Med. 194: 1171-1178
    3. Shortman, K. and Liu, Y. J. (2002) Mouse and human dendritic cell subtypes. Nat. Rev. Immunol. 2: 151-161
    4. Fischer et al. (2004) MACS&more 8(1): 13-15
Product options: 1
for 2×
10
9
total cells
GBP 560.00

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