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MDCs were isolated from human PBMCs using the Myeloid Dendritic Cell Isolation Kit. Cells were fluorescently stained with CD141 (BDCA- 3)-FITC, CD20-PE, CD14-VioBlue, and CD1c (BDCA-1)-APC and analyzed by flow cytometry. Exclusion of CD1c+
B cells and CD14+
monocytes was performed. Shown are CD1c (BDCA-1) and CD141 (BDCA-3) stainings of total cells before and after separation.
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