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Isolation of CD304 (BDCA-4/Neuropilin-1)+
plasmacytoid dendritic cells from PBMCs using the CD304 (BDCA-4/Neuropilin-1) MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were stained with CD303 (BDCA-2)-FITC and CD123-APC. Like CD304 (BDCA-4/Neuropilin-1), the CD303 (BDCA-2) antigen is specifically expressed on plasmacytoid dendritic cells in blood, and allows their direct identification. Apart from plasmacytoid dendritic cells, CD123 is also expressed at high levels on basophils and at low levels on monocytes and myeloid dendritic cells.
PBMCs before separation
Enriched CD304 (BDCA-4/Neuropilin-1)+
plasmacytoid blood dendritic cells
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