Mouse IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Mouse IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE)

The IL-2 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IL-2-secreting cells.

Background information

Interleukin-2 (IL-2) is a T cell-derived cytokine known to promote growth and differentiation of T cells and has pleiotropic effects on many other leukocytes. IL-2 is rapidly secreted upon activation by naive T helper cells and certain subsets of memory T cells.

Downstream applications

The Mouse IL-2 Secretion Assay was used to study the sequential production of cytokines by T helper lymphocytes.
Furthermore, it was used for isolation and analysis of IL-2-secreting NKT cells after stimulation with glycolipids.
The IL-2 Secretion Assay has also been used as a highly sensitive read-out system for peptide-specific T cells in TCR transgenic mice.
T cell suppression by CD4
regulatory T cells has been analyzed with the IL-2 Secretion Assay by coculturing responder T cells with regulatory T cells (distinguished by Thy1.1 and Thy1.2 expression) followed by cytokine detection and costaining with Thy1.1.


MS, LS, or autoMACS
  • Selected references

    1. Assenmacher et al. (1998) Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes. Eur. J. Immunol. 28: 1534-1543
    2. Yang, J. Q. et al. (2003) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis. J. Immunol. 171: 2142-2153
    3. Sojka, D. K. et al. (2004)
      IL-2 secretion by CD4
      T cells
      in vivo
      is rapid, transient, and influenced by TCR-specific competition.
      J. Immunol. 172: 6136-6143
    4. Sojka, D. K. et al. (2005) Early kinetic window of target T cell susceptibility to CD25+ regulatory T cell activity. J. Immunol. 175: 7274-7280
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