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Untouched eosinophils were isolated from 30 mL of human EDTA anticoagulated whole blood using the MACSxpress®
Whole Blood Eosinophil Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with Anti-Siglec-8-APC, CD45-VioBlue®
and CD15-PE and analyzed by flow cytometry using the MACSQuant®
Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
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