PepTivator PRAME, human

PepTivator PRAME, human

PRAME is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the preferentially expressed antigen of melanoma (PRAME) (UniProt ID: P78395).
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Background information

PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through certain retinoic acid receptors. Moreover, it is a melanoma antigen aberrantly expressed in chronic lymphocytic leukemia and mantle cell lymphoma.

Downstream applications

PepTivator PRAME – research grade has been specifically developed for efficient
in vitro
stimulation of PRAME –specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
and CD8
T cells in various applications, including:
  • Detection and analysis of PRAME-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable PRAME-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of PRAME-specific CD4+ and CD8+ effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.
Product options: 1
stimulation of
GBP 135.00

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