Human IL-1β

Human IL-1β

IL-1β stands for interleukin-1 beta. Human IL-1β is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-1β can be used for a variety of applications including:
  • Induction of Mo-DC maturation.
  • Chemotaxis assays.
  • Investigation of IL-1 receptor signaling.

Alternative names

IL1F2

Background information

Interleukin 1β (IL-1β) is a proinflammatory cytokine that is secreted mainly by monocytes and macrophages. IL-1β secretion has also been reported for a variety of other cells, including B cells, NK cells, dendritic cells, astrocytes, and microglial cells. It mediates inflammatory responses in B cells, T cells, and NK cells by inducing the production of cytokines, such as IL-2, IL-3, IL-6, as well as interferons. Upon exposure to IL-1β, endothelial cells and smooth muscle cells synthesize prostaglandins and other derivatives of arachidonic acid. In addition, IL-1β is found in synovial fluid of arthritis patients, causing degranulation of basophils and eosinophils as well as activation of osteoclasts. IL-1β is mitogenic for mesangial cells, glial cells, and keratinocytes. It has been shown that IL-1β is a potent modulator of CD40L-induced cytokine secretion by human dendritic cell (DC) subsets, such as monocyte-derived dendritic cells (MoDCs), CD34
+
-derived DCs, and peripheral blood DCs.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of D10.G4.1 cells (NIBSC 86/680)
  • premium grade: ≥ 3×
    10
    7
    IU/mg
  • research grade: ≥ 1×
    10
    7
    IU/mg
  • Selected references

    1. Cunin P. et al. (2011) The tachykinins substance P and hemokinin-1 favor the generation of human memory Tʜ17 cells by inducing IL-1β, IL-23, and TNF-like 1A expression by monocytes. J. Immunol. 186(7): 4175-4182
    2. Poole, S. and Gaines-Das, R. E. (1991) The international standards for interleukin-1 alpha and interleukin-1 beta. Evaluation in an international collaborative study. J. Immunol. Methods 142: 1-13
    3. Dussiau C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
    4. Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928
    5. Schipper H. S. et al. (2010) A multiplex immunoassay for human adipokine profiling. Clin. Chem. 56(8): 1320-1328
    6. Kitamura, T. et al. (1991) IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoetic cell line, TF-1. Int. Immunol. 3: 571-577
    7. Luft, T. et al. (2002) IL-1β enhances CD40 ligand-mediated cytokine secretion by human dendritic cells (DC): a mechanism for T cell-independent DC activation. J. Immunol. 168: 713-722
Product options: 6
E. coli
10 µg
GBP 130.00
E. coli
25 µg
GBP 245.00
E. coli
10 µg
GBP 160.00
E. coli
25 µg
GBP 305.00
E. coli
100 µg
GBP 530.00
E. coli
1000 µg
GBP 2,335.00

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