Vio Bright V423 for violet lasers
Vio Bright V423 is Miltenyi Biotec’s brightest dye excited at 405 nm. In addition to high brightness this dye shows a significantly improved signal-to-noise ratio, minimal intralaser compensation and a superior detection of rare antigens.
Vio Bright V423 is a synthetic dye which is 5-times brighter compared to the VioBlue® Dye. This makes Vio Bright V423 an excellent choice for multiparameter cell analysis.
| Laser | Violet (405 nm) |
| Excitation max | 399 nm |
| Emission max | 420 nm |
| Application | Flow cytometry |
| Common filter set (FC) | 450/50 nm |
| Stain index | 5 out of 5 |
| Photostability | Photostable to ambient light |
| Fixation stability | PFA |
Vio Bright V423 is a superior alternative to:
- Brilliant Violet™ 421
- Super Bright 436
Brighter and clearer staining for violet laser
- Enhanced or similar brightness compared to Brilliant Violet 421 and Super Bright 421
- Enhanced brightness of Vio Bright V423 conjugates across different antigens
High specificity of fluorescent signal
- Exceptional brightness allows for staining of rare cells and dim markers, e.g. CD25
- Even better brightness than Vio Bright B515
Decreased spillover
- Minimal spillover into neighbouring detection channels
- Minimal change in compensation settings required
Compensation matrix for a CD19 antibody conjugated to different fluorochromes excited by a violet laser. Values depict the percentage of compensation required for the respective detection filter.
| Conjugate | V1-A (450/50nm) | V2-A (525/50 nm) | V3-A (579/34 nm) | V4-A (615/20 nm) | V5-A (667/30 nm) | |
|---|---|---|---|---|---|---|
| V1-A (450/50 nm) | CD19-Vio Bright V423 | 100 | 4 | 1 | ||
| V2-A (525/50 nm) | CD19-BV510 | 40 | 100 | 60 | 40 | 6 |
| V3-A (579/34 nm) | CD19-BV570 | 40 | 3 | 100 | 65 | 10 |
| V5-A (667/30 nm) | CD19-BV650 | 30 | 3 | 52 | 100 |
Excellent fixation stability
- Excellent stability after fixation with paraformaldehyde
- Compatible with different surface and intracellular staining protocols and buffer sets
- Conjugate performance may vary depending on antigen nature upon fixation
High photostability
- High photostability
- Only minor drop in mean fluorescent intensity (MFI) and stain index (SI) after 24 h at continuous exposure to ambient light (~850 lux)
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