Vio Bright V423 is Miltenyi Biotec’s brightest dye excited at 405 nm. In addition to high brightness this dye shows a significantly improved signal-to-noise ratio, minimal intralaser compensation and a superior detection of rare antigens.
Vio Bright V423 is a synthetic dye which is 5-times brighter compared to the VioBlue® Dye. This makes Vio Bright V423 an excellent choice for multiparameter cell analysis.
Laser | Violet (405 nm) |
Excitation max | 399 nm |
Emission max | 420 nm |
Application | Flow cytometry |
Common filter set (FC) | 450/50 nm |
Stain index | 5 out of 5 |
Photostability | Photostable to ambient light |
Fixation stability | PFA |
Compensation matrix for a CD19 antibody conjugated to different fluorochromes excited by a violet laser. Values depict the percentage of compensation required for the respective detection filter.
Conjugate | V1-A (450/50nm) | V2-A (525/50 nm) | V3-A (579/34 nm) | V4-A (615/20 nm) | V5-A (667/30 nm) | |
---|---|---|---|---|---|---|
V1-A (450/50 nm) | CD19-Vio Bright V423 | 100 | 4 | 1 | ||
V2-A (525/50 nm) | CD19-BV510 | 40 | 100 | 60 | 40 | 6 |
V3-A (579/34 nm) | CD19-BV570 | 40 | 3 | 100 | 65 | 10 |
V5-A (667/30 nm) | CD19-BV650 | 30 | 3 | 52 | 100 |
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