Vio Bright V423 is Miltenyi Biotec’s brightest dye excited at 405 nm. In addition to high brightness this dye shows a significantly improved signal-to-noise ratio, minimal intralaser compensation and a superior detection of rare antigens.
Vio Bright V423 is a synthetic dye which is 5-times brighter compared to the VioBlue® Dye. This makes Vio Bright V423 an excellent choice for multiparameter cell analysis.
|Laser||Violet (405 nm)|
|Excitation max||399 nm|
|Emission max||420 nm|
|Common filter set (FC)||450/50 nm|
|Stain index||5 out of 5|
|Photostability||Photostable to ambient light|
Compensation matrix for a CD19 antibody conjugated to different fluorochromes excited by a violet laser. Values depict the percentage of compensation required for the respective detection filter.
|V1-A (450/50 nm)||CD19-Vio Bright V423||100||4||1|
|V2-A (525/50 nm)||CD19-BV510||40||100||60||40||6|
|V3-A (579/34 nm)||CD19-BV570||40||3||100||65||10|
|V5-A (667/30 nm)||CD19-BV650||30||3||52||100|
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