Clone:
REA1017
Type of antibody:
Secondary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, 3D-IF

Extended validation for IgG1 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1017
X56++
RMG1-1++
A85-1++
M1-14D12-
Cells were incubated with an excess of purified unconjugated IgG1 (REA1017) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for IgG1. Human peripheral blood mononuclear cells (PBMCs) inkubated with CD4 pure antibodies were stained with IgG1 antibodies and with a suitable counterstaining. As a control, IgG1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IgG1. Human peripheral blood mononuclear cells (PBMCs) inkubated with CD4 pure antibodies were stained with IgG1 antibodies and with a suitable counterstaining. As a control, IgG1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IgG1. Human peripheral blood mononuclear cells (PBMCs) inkubated with CD4 pure antibodies were stained with IgG1 antibodies and with a suitable counterstaining. As a control, IgG1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IgG1. Human peripheral blood mononuclear cells (PBMCs) inkubated with CD4 pure antibodies were stained with IgG1 antibodies and with a suitable counterstaining. As a control, IgG1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IgG1. Human peripheral blood mononuclear cells (PBMCs) inkubated with CD4 pure antibodies were stained with IgG1 antibodies and with a suitable counterstaining. As a control, IgG1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for IgG1. Human peripheral blood mononuclear cells (PBMCs) inkubated with CD4 pure antibodies were stained with IgG1 antibodies and with a suitable counterstaining. As a control, IgG1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using IgG1 (REA1017). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using IgG1 (REA1017). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using IgG1 (REA1017). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using IgG1 (REA1017). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using IgG1 (REA1017). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for IgG1 Antibody, anti-mouse, REAfinity™

Overview

Clone REA1017 is suited for flow cytometric analysis of mouse B cells expressing surface IgG1, for example, memory B cells. The antibody can further be used for the indirect immunofluorescent staining and flow cytometric analysis of cells stained with primary mouse antibodies of IgG1 isotype.
Additional information: Clone REA1017 displays negligible binding to Fc receptors.

Detailed product information

Technical specifications

CloneREA1017
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodySecondary antibodies, Recombinant antibodies
Speciesmouse
AntigenIgG1
Molecular mass of antigen [kDa]36
Distribution of antigenB cells, memory B cells
Entrez Gene ID16017
RRIDAB_2733864, AB_2733865, AB_2733425, AB_2733426, AB_2733962, AB_2733963, AB_2734102, AB_2734103, AB_2732998, AB_2732999, AB_2733264, AB_2733265, AB_2733155, AB_2733055, AB_2733056, AB_2733920, AB_2733921, AB_2734039, AB_2734040, AB_2733922, AB_2733923, AB_2733550, AB_2733551, AB_2905275, AB_2733673

Resources for IgG1 Antibody, anti-mouse, REAfinity™

Certificates

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