Recombinant human IL-3 (interleukin 3) can promote proliferation, survival, and differentiation of hematopoietic stem cells and committed progenitor cells of the myeloid or megakaryocyte lineage. This includes cells of the basophilic, megakaryocyte, erythroid, eosinophilic, monocytic, and mast cell lineage. Suitable for use in cell culture, functional assays, and differentiation studies, the recombinant human IL-3 is ideal for research.

Data and images for Human IL-3

Figures

Figure 1

View details
SDS-PAGE of Human IL-3, premium grade
from Miltenyi Biotec (MB) in comparison to recombinant human IL-3 from a different supplier (S), both under reducing and non-reducing conditions.

Figure 1

SDS-PAGE of Human IL-3, premium grade
from Miltenyi Biotec (MB) in comparison to recombinant human IL-3 from a different supplier (S), both under reducing and non-reducing conditions.

Figure 2

View details
Mass spectrometry analysis (ESI-MS) of Human IL-3, premium grade. The peak corresponds to the calculated molecular mass of 15089 Da.

Figure 2

Mass spectrometry analysis (ESI-MS) of Human IL-3, premium grade. The peak corresponds to the calculated molecular mass of 15089 Da.

Specifications for Human IL-3

Overview

Recombinant human IL-3 (interleukin 3) can promote proliferation, survival, and differentiation of hematopoietic stem cells and committed progenitor cells of the myeloid or megakaryocyte lineage. This includes cells of the basophilic, megakaryocyte, erythroid, eosinophilic, monocytic, and mast cell lineage. Suitable for use in cell culture, functional assays, and differentiation studies, the recombinant human IL-3 is ideal for research.

Applications

Human IL-3 can be used for a variety of applications, including:
  • Induction of colony formation from hematopoietic progenitor cells in semi-solid medium in vitro, for example, CD34+ cells from umbilical cord blood2.
  • In vitro differentiation studies, for example, of B lymphoid progenitors3.
  • Cultivation of plasmacytoid dendritic cells4.
  • In vitro expansion of hematopoietic stem cells.
  • Investigation of mast cell or basophil function, for example, basophil interaction with blood vessels5.
  • Investigation of IL-3–mediated signaling pathways.

Detailed product information

Background information

Interleukin 3 (IL-3) is a hematopoietic growth factor, which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

Biological activity

  • Proliferation of TF-1 cells (NIBSC 91/510)
  • research grade: ≥ 1×
    10
    6
    IU/mg
  • premium grade: ≥ 2×
    10
    6
    IU/mg
    (Typical specific activity: ≥ 2.3×
    10
    6
    IU/mg
    )
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IL-3

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-3

Publications

  1. Xie, Z. S. et al. (2019) Sphingolipid Modulation Activates Proteostasis Programs to Govern Human Hematopoietic Stem Cell Self-Renewal. Cell Stem Cell 25(5): 639-653
  2. Bianchi, E. et al. (2015) MYB controls erythroid versus megakaryocyte lineage fate decision through the miR-486-3p-mediated downregulation of MAF. Cell Death Differ. 22(12): 1906-1921
  3. Youn, M. et al. (2017) Loss of Forkhead box M1 promotes erythropoiesis through increased proliferation of erythroid progenitors. Haematologica 102(5): 826-834
  4. Munitión, S. et al. (2016) Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients. PLoS One 11(2): e0146722
  5. Moussy, A. et al. (2017) Integrated time-lapse and single-cell transcription studies highlight the variable and dynamic nature of human hematopoietic cell fate commitment. PLoS Biol. 15(7): e2001867
  6. Aires, V. et al. (2021) CD22 Blockage Restores Age-Related Impairments of Microglia Surveillance Capacity. Front Immunol 12: 684430
  7. Cassuto, O. et al. (2012) All tyrosine kinase inhibitor-resistant chronic myelogenous cells are highly sensitive to ponatinib. Oncotarget. 3(12): 1557-1567
  8. Chae, H.-D. et al. (2020) RSK inhibitor BI-D1870 inhibits acute myeloid leukemia cell proliferation by targeting mitotic exit. Oncotarget. 11(25): 2387-2403
  9. Nakazawa, Y. et al. (2016) Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34(+) cells of juvenile myelomonocytic leukemia. J Hematol Oncol. 9: 27
  10. Geeraerts, S. L. et al. (2021) Repurposing the Antidepressant Sertraline as SHMT Inhibitor to Suppress Serine/Glycine Synthesis-Addicted Breast Tumor Growth. Mol Cancer Ther. 20(1): 50-63
  11. Cordes, N. et al. (2021) Anti-CD19 CARs displayed at the surface of lentiviral vector particles promote transduction of target-expressing cells. Mol Ther Methods Clin Dev. 21: 42-53
  12. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
  13. Rossmanith, T. et al. (2001)
    Interleukin 3 improves the
    ex vivo
    expansion of primitive human cord blood progenitor cells and maintains the engraftment potential of SCID repopulating cells.
    Stem Cells 19: 313-320
  14. Crooks, G. M. et al. (2000)
    IL-3 increases production of B lymphoid progenitors from human CD34
    +
    CD38
    cells.
    J. Immunol. 165: 2382-2389
  15. Tas, S. W. et al. (2007) Noncanonical NF-kappaB signaling in dendritic cells is required for indoleamine 2,3-dioxygenase (IDO) induction and immune regulation. Blood 110: 1540-1549
  16. Lim, L. H. et al. (2006)
    Stimulation of human endothelium with IL-3 induces selective basophil accumulation
    in vitro.
    J. Immunol. 176: 5346-5353
  17. Jing, D. et al. (2010)
    Hematopoietic stem cells in co-culture with mesenchymal stromal cells--modeling the niche compartments
    in vitro
    .
    Haematologica 95(4): 542-550
  18. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
  19. Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
  20. Brault, J. et al. (2014)
    Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
    ex vivo
    models of X
    0
    -linked, AR22
    0
    - and AR47
    0
    - chronic granulomatous diseases.
    Biores Open Access 3(6): 311-326
  21. Dighe, N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
  22. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313

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