IL-13 stands for interleukin 13. Human IL-13 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human IL-13

Figures

Figure 1

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SDS-PAGE of Human IL-13, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 1

SDS-PAGE of Human IL-13, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

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Mass spectrometry analysis (ESI-MS) of Human IL-13, premium grade. The peak corresponds to the calculated molecular mass of 12524 Da.

Figure 2

Mass spectrometry analysis (ESI-MS) of Human IL-13, premium grade. The peak corresponds to the calculated molecular mass of 12524 Da.

Specifications for Human IL-13

Overview

IL-13 stands for interleukin 13. Human IL-13 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-13 can be used for a variety of applications, including:
  • Inhibition of monocyte and macrophage activation.
  • B cell activation.
  • NK cell and LAK cell stimulation.

Detailed product information

Background information

Interleukin 13 (IL-13) is an immunoregulatory protein, which shares similarity in structure and function with IL-4. IL-13 is mainly secreted by Tʜ2 cells, as well as mast cells and NK cells, and binds a multi-subunit receptor, including the alpha chain of the IL-4 receptor, which downstream activates the signal transducer and activator of transcription 6 (STAT-6). Although IL-13 is involved in the pathogenesis of airway diseases such as asthma, it also plays a protective role for the mucosa, via induction of matrix metalloproteinases (MMPs) and other anti-inflammatory effects. Similarly to IL-4, IL-13 induces humoral responses favoring B cell proliferation and isotype switching to IgE, whereas, on monocytes and macrophages, it counteracts the effects of IL-1β, TNF-α, IL-6, by inhibiting the secretion of inflammatory cytokines and increasing IL-1RA expression. IL-13 acts on hematopoietic differentiation and can synergize with IL-2 in the activation of IFN-γ mediated killer activity of lymphokine activated killer (LAK) cells. The protein sequences of human and mouse IL-13 share 58% identity and are cross species reactive.

Biological activity

  • Proliferation of TF-1 cells (NIBSC 94/622)
  • research grade: ≥ 2×
    10
    5
    U/mg
  • premium grade: ≥ 4×
    10
    5
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IL-13

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-13

Publications

  1. Menegatti, S. et al. (2020) Immune response profiling of patients with spondyloarthritis reveals signalling networks mediating TNF-blocker function in vivo. Ann. Rheum. Dis. 80(4): 475-486
  2. Acciani, T. H. et al. (2016) Epidermal growth factor receptor signalling regulates granulocyte-macrophage colony-stimulating factor production by airway epithelial cells and established allergic airway disease. Clin. Exp. Allergy 46(2): 317-328
  3. Liaskou, E. et al. (2013) Monocyte subsets in human liver disease show distinct phenotypic and functional characteristics. Hepatology 57(1): 385-398
  4. Segan, S. et al. (2020) Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro. Biomed Res Int 2020: 3481549
  5. Braile, M. et al. (2021) Human Lung-Resident Macrophages Express and Are Targets of Thymic Stromal Lymphopoietin in the Tumor Microenvironment. Cells 10(8): 2012
  6. Kasper, J. Y. et al. (2017) A responsive human triple-culture model of the air-blood barrier: incorporation of different macrophage phenotypes. J Tissue Eng Regen Med 11(4): 1285-1297
  7. Dilasser, F. et al. (2021) Essential role of smooth muscle Rac1 in severe asthma-associated airway remodelling. Ther Clin Risk Manag 76(4): 326-334
  8. Diedrich, C. R. et al. (2013) Monocyte-derived IL-5 reduces TNF production by Mycobacterium tuberculosis-specific CD4 T cells during SIV/M. tuberculosis coinfection. J. Immunol. 190(12): 6320-6328
  9. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
  10. Borriello, F. et al. (2015) IL-3 synergizes with basophil-derived IL-4 and IL-13 to promote the alternative activation of human monocytes. Eur. J. Immunol. 45(7): 2042-2051

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