Clone:
REA900
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
KARAP, TYROBP

Extended validation for DAP12 Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for DAP12. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining followed by a fixation and permeabilization step using Inside Stain Kit. Cells were then stained with DAP12 antibodies. As a control, DAP12 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for DAP12. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining followed by a fixation and permeabilization step using Inside Stain Kit. Cells were then stained with DAP12 antibodies. As a control, DAP12 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for DAP12. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining followed by a fixation and permeabilization step using Inside Stain Kit. Cells were then stained with DAP12 antibodies. As a control, DAP12 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for DAP12. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining followed by a fixation and permeabilization step using Inside Stain Kit. Cells were then stained with DAP12 antibodies. As a control, DAP12 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for DAP12. Human peripheral blood mononuclear cells (PBMCs) were stained with a suitable counterstaining followed by a fixation and permeabilization step using Inside Stain Kit. Cells were then stained with DAP12 antibodies. As a control, DAP12 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for DAP12 Antibody, anti-human, REAfinity™

Overview

Clone REA900 recognizes the human DAP12 antigen, a transmembrane signaling polypeptide which consists of an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. DAP12 interacts with several members of the CD300 molecule-like family, CLECSF5 and Siglec14. The association of DAP12 with triggering receptor expressed on myeloid cells (TREM-1) and myeloid DAP12-associating lectin (MDL-1) plays a role in monocytic activation and inflammatory response. DAP12 is found in several tissues, but also in peripheral blood leukocytes.
Additional information: Clone REA900 displays negligible binding to Fc receptors.

Alternative names

KARAP, TYROBP

Detailed product information

Technical specifications

CloneREA900
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenDAP12
Alternative names of antigenKARAP, TYROBP
Molecular mass of antigen [kDa]10
Distribution of antigenleukocytes, monocytes, other, brain, spleen
Entrez Gene ID7305
RRIDAB_2726906, AB_2726872, AB_2726907, AB_2726873, AB_2889656, AB_2889655

Resources for DAP12 Antibody, anti-human, REAfinity™

Certificates

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References for DAP12 Antibody, anti-human, REAfinity™

Publications

  1. Tomasello, E. et al. (2000) Association of signal-regulatory proteins beta with KARAP/DAP-12. Eur. J. Immunol. 30(8): 2147-2156
  2. Gingras, M. S. et al. (2002) TREM-1, MDL-1, and DAP12 expression is associated with a mature stage of myeloid development. Mol. Immunol. 38(11): 817-824

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