Colon TumorMACS Medium is an optimized serum-free cell culture medium for the cultivation and expansion of tumor cells from primary and xenotransplanted colorectal tumors. It enables the establishment of new cell lines from freshly dissociated tumor tissue.

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Figure 1

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Primary human colon carcinoma cells were cultivated and propagated in vitro using Colon TumorMACS Medium directly after the dissociation of a tumor biopsy using the gentleMACS. The medium supported cell expansion both in adherent and 3D cultures.
This is of particular interest, as the data show that the epithelial tumor cells retain their potential to form spheroid-like structures. Also the morphological heterogeneity could be preserved up to late passages (> p20). 10x Magnification.

Figure 1

Primary human colon carcinoma cells were cultivated and propagated in vitro using Colon TumorMACS Medium directly after the dissociation of a tumor biopsy using the gentleMACS. The medium supported cell expansion both in adherent and 3D cultures.
This is of particular interest, as the data show that the epithelial tumor cells retain their potential to form spheroid-like structures. Also the morphological heterogeneity could be preserved up to late passages (> p20). 10x Magnification.

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Overview

Colon TumorMACS Medium is an optimized serum-free cell culture medium for the cultivation and expansion of tumor cells from primary and xenotransplanted colorectal tumors. It enables the establishment of new cell lines from freshly dissociated tumor tissue.

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Background information

The Colon TumorMACS Medium is an optimized and standardized medium for the reliable expansion of tumor cells from primary or xenotransplanted human colorectal tumor samples. Tumor cells from freshly dissociated tissue can be cultured over multiple passages, allowing for in vitro assays, such as drug screenings on primary cell cultures or characterization studies including gene expression or protein profiling. In addition, the Colon TumorMACS Medium supports the establishment of new cell lines and thereby obtaining sufficient cell numbers for further experiments, such as in vivo transplantation studies in animals. Colon TumorMACS Medium efficiently expands cultures of primary colorectal tumor cells in vitro while maintaining the cell populations' heterogeneity and tumorigenic potential. The medium is manufactured under strictly controlled conditions using ingredients of the highest quality. Colon TumorMACS Medium offers consistent lot-to-lot performance and optimal conditions for the cultivation of primary colorectal tumor cells.

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