MACS GMP Recombinant Human TPO

MACS GMP Recombinant Human TPO

Thrombopoietin (TPO), also termed megakaryocyte growth and development factor (MGDF), is a glycoprotein hormone and the major stimulator of megakaryopoiesis and platelet production. It promotes the proliferation of hematopoietic stem cells, primitive progenitors, megakaryocytes, and platelets.
MACS GMP Recombinant Human TPO is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product. The product is lyophilized without carrier protein or preservatives.


MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Background information

TPO is expressed in liver, kidney, spleen, lung, bone marrow, and brain. The TPO receptor is a product of the proto-oncogene
and displays homology with type I cytokine receptor superfamily members. The receptor is present mainly on hematopoietic stem cells, megakaryocytic progenitors, megakaryocytes, and platelets.


MACS GMP Recombinant Human TPO can be used for a variety of applications, including the expansion
and the viral transduction
of hematopoietic stem and progenitor cells derived from bone marrow, peripheral blood cells, and cord blood, or the
in vitro
differentiation of megakaryocytes

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Biological activity

  • The specific activity is determined by proliferation assay according to Page
    et al.1
    using M07e cells. The proliferation assay was calibrated with the Non WHO Reference Material for human TPO (NIBSC code 03/124) provided by the National Institute for Biological Standards and Control.
  • Specific activity: ≥ 1×
  • Selected references

    1. Page, L. A. et al. (1996) A sensitive human cell line based bioassay for megakaryocyte growth and development factor or thrombopoietin. Cytokine 8(1): 66-69
    2. Kaushansky, K. and Drachman, J. G. (2002) The molecular and cellular biology of thrombopoietin: the primary regulator of platelet production. Oncogene 21(21): 3359-3367
    3. Petzer, A. L. et al. (1996)
      Differential cytokine effects on primitive (CD34
      ) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.
      J. Exp. Med. 183(6): 2551-2558
    4. Kiem, H. P. et al. (1998) Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor. Blood 92(6): 1878-1886
    5. Uchida, N. et al. (2011)
      Optimal conditions for lentiviral transduction of engrafting human CD34
      Gene Ther. 18(11): 1078-1086
    6. Bruno, S. et al. (2003)
      In vitro
      in vivo
      megakaryocyte differentiation of fresh and
      expanded cord blood cells: rapid and transient megakaryocyte reconstitution.
      Haematologica 88(4): 379-387
  • Brochures and posters

  • Customer reports and application notes

  • Certificates

    Please follow this
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 1
E. coli
50 µg
GBP 840.00

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