MACS GMP Recombinant Human TNF-α

MACS GMP Recombinant Human TNF-α

Tumor necrosis factor alpha (TNF-α) has a broad spectrum of biological activities. In addition to its central role in inflammation TNF-α is involved in regulating apoptotic cell death, cellular proliferation, and differentiation.
MACS GMP Recombinant Human TNF-α is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.


MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Background information

TNF-α is mainly produced by activated monocytes and macrophages, but also by a broad variety of other cell types including lymphoid cells, mast cells, endothelial cells, fibroblasts, and astrocytes. Beside its primary role in immune regulation, TNF-α promotes angiogenesis, bone resorption, and thrombotic processes.


MACS GMP Recombinant Human TNF-α can be used for a variety of applications, including the
ex vivo
generation of human dendritic cells from enriched CD14
monocyte populations.

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Biological activity

  • The specific activity is determined by cytotoxicity assay using L929 cells provided by the German Collection of Microorganisms and Cell Cultures (DSMZ) in the presence of 1 μg/mL actinomycin D according to Baarsch
    et al
    The cytotoxicity assay was calibrated with the international standard for human TNF-α (NIBSC code 88/786) provided by the National Institute for Biological Standards and Control.
  • Specific activity: ≥ 2×
  • Selected references

    1. Baarsch, M. J. et al. (1991) Detection of tumor necrosis factor alpha from porcine alveolar macrophages using an L929 fibroblast bioassay. J. Immunol. Methods 140: 15-22
    2. Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
    3. Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
    4. Jonuleit et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142
  • Brochures and posters

  • Certificates

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    to search for Certificates of Analysis (CoA) by lot number.
Product options: 2
E. coli
25 µg
GBP 360.00
E. coli
100 µg
GBP 1,155.00