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Human EDTA-treated whole blood samples were pre-washed to remove soluble immuno globulins and stained with Anti-IgD antibodies as well as CD19 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant®
Analyzer. For analysis, lymphocytes were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.
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