Clone:
PO3.3
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC, MICS, IF, IHC
Alternative names:
B7-2, B70, CLS1, Cd28l2, ETC-1, Ly-58, MB7-2, TS/A-2

Extended validation for CD86 Antibody, anti-mouse

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD86. A mixture of CD11c (+) stimulated with 100ng/ml LPS for 24 hours and CD11c (-) unstimulated Balb/c Splenocytes were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. A mixture of CD11c (+) stimulated with 100ng/ml LPS for 24 hours and CD11c (-) unstimulated Balb/c Splenocytes were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. A mixture of CD11c (+) stimulated with 100ng/ml LPS for 24 hours and CD11c (-) unstimulated Balb/c Splenocytes were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. A mixture of CD11c (+) stimulated with 100ng/ml LPS for 24 hours and CD11c (-) unstimulated Balb/c Splenocytes were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. A mixture of CD11c (+) stimulated with 100ng/ml LPS for 24 hours and CD11c (-) unstimulated Balb/c Splenocytes were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. A mixture of CD11c (+) stimulated with 100ng/ml LPS for 24 hours and CD11c (-) unstimulated Balb/c Splenocytes were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. A mixture of CD11c (+) stimulated with 100ng/ml LPS for 24 hours and CD11c (-) unstimulated Balb/c Splenocytes were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. A mixture of CD11c (+) stimulated with 100ng/ml LPS for 24 hours and CD11c (-) unstimulated Balb/c Splenocytes were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD86. A mixture of CD11c (+) stimulated with 100ng/ml LPS for 24 hours and CD11c (-) unstimulated Balb/c Splenocytes were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD86 (PO3.3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD86 (PO3.3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD86 (PO3.3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD86 Antibody, anti-mouse

Overview

Antibody clone PO3.3 detects the CD86 antigen, also known as B7-2, an 80 kDa molecule and a member of the immunoglobulin superfamily. Together with CD80 (B7-1) it belongs to the B7 family of costimulatory molecules. CD86 is expressed on activated antigen-presenting cells, including B cells, dendritic cells, and monocytes/macrophages. The interaction of CD86 with its ligands CD28 and CD152 (CTLA- 4) plays a critical role in induction and regulation of immune responses, e.g., cross-talk between T and B cells, T cell costimulation, or immunoglobulin class-switching. Binding of CD86 to CD28 on T cells results in transduction of costimulatory signals for activation or proliferation of T cells, or cytokine production. In contrast, binding of CD86 to CTLA-4 regulates T cell activation and diminishes the immune response.

Alternative names

B7-2, B70, CLS1, Cd28l2, ETC-1, Ly-58, MB7-2, TS/A-2

Detailed product information

Technical specifications

ClonePO3.3
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD86
Alternative names of antigenB7-2, B70, CLS1, Cd28l2, ETC-1, Ly-58, MB7-2, TS/A-2
Molecular mass of antigen [kDa]32
Distribution of antigenB cells, dendritic cells, endothelial cells, Langerhans cells, monocytes, T cells
Entrez Gene ID12524
RRIDAB_2889634, AB_2811485, AB_2811413, AB_2660747, AB_2660749, AB_2660750, AB_2660753, AB_2660754, AB_2660755, AB_2889633

Resources for CD86 Antibody, anti-mouse

Certificates

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References for CD86 Antibody, anti-mouse

Publications

  1. Kin, N. W. et al. (2006) CD86 stimulation on a B cell activates the phosphatidylinositol 3-kinase/Akt and phospholipase C gamma 2/protein kinase C alpha beta signaling pathways. J. Immunol. 176(11): 6727-6735
  2. Nakajima, A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells. Int. Immunol. 9(5): 637-644
  3. Zhu, X. Y. et al. (2005)
    Blockade of CD86 signaling facilitates a Tʜ2 bias at the maternal-fetal interface and expands peripheral CD4
    +
    CD25
    +
    regulatory T cells to rescue abortion-prone fetuses.
    Biol. Reprod. 72(2): 338-345

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