Clone:
DF-T1
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
Spn, Leukosialin, GALGP, gpL115, Lsn

Extended validation for CD43 Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD43. Human peripheral blood mononuclear cells (PBMCs) were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Human peripheral blood mononuclear cells (PBMCs) were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Human peripheral blood mononuclear cells (PBMCs) were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Human peripheral blood mononuclear cells (PBMCs) were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Human peripheral blood mononuclear cells (PBMCs) were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Human peripheral blood mononuclear cells (PBMCs) were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Human peripheral blood mononuclear cells (PBMCs) were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD43. Human peripheral blood mononuclear cells (PBMCs) were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD43 (DF-T1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD43 (DF-T1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD43 (DF-T1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD43 Antibody, anti-human

Overview

Human CD43 (leukosialin) is a cell surface sialoglycoprotein that is involved in cell adhesion, differentiation and activation. The CD43 antigen is expressed on most leukocytes, i.e., T cells, NK cells, granulocytes, monocytes, and macrophages, on hematopoietic stem cells, and on platelets, but not on erythrocytes. Among B cells, CD43 is expressed on activated B cells and plasma cells but not on resting B cells, e.g., naive B cells. In bone marrow, CD43 is found on pro-B cells but is down-regulated during transition to the pre-B cell stage. CD43 is expressed on different myeloid and lymphoid tumors, including some B cell malignancies.

Alternative names

Spn, Leukosialin, GALGP, gpL115, Lsn

Detailed product information

Technical specifications

CloneDF-T1
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
olive baboon (
Papio anubis
)
AntigenCD43
Alternative names of antigenSpn, Leukosialin, GALGP, gpL115, Lsn
Molecular mass of antigen [kDa]38
Distribution of antigenB cells, granulocytes, macrophages, megakaryocytes, T cells, monocytes, NK cells, platelets, red blood cells, lymphocytes, stem cells, basophils, mesenchymal stem cells, neutrophils, plasma cells, thymocytes, bone marrow
Entrez Gene ID6693
RRIDAB_2819468, AB_2876908, AB_2876909, AB_2658125, AB_2658126, AB_2658127, AB_2658128, AB_2658131, AB_2658136, AB_2658137, AB_2658138, AB_2658139, AB_2819480

Resources for CD43 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD43 Antibody, anti-human

Publications

  1. Stross, W. P. et al. (1989) Molecule detected in formalin fixed tissue by antibodies MT1, DF-T1, and L60 (Leu-22) corresponds to CD43 antigen. J. Clin. Pathol. 42: 953-961

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