Clone:
REA185
Type of antibody:
Primary antibodies, Recombinant antibodies, Functional-grade antibodies
Isotype:
recombinant human IgG1
Applications:
FC, FA
Alternative names:
GP1BA, BDPLT1, BDPLT3, BSS, CD42b-alpha, DBPLT3, GP1B, GPIbA, VWDP, gpIbα

Extended validation for CD42b Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA185
REAL344++
HIP1+
Cells were incubated with an excess of purified unconjugated CD42b (REA185) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD42b. Human platlets were stained with CD42b antibodies and with a suitable counterstaining. As a control, CD42b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD42b. Human platlets were stained with CD42b antibodies and with a suitable counterstaining. As a control, CD42b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD42b. Human platlets were stained with CD42b antibodies and with a suitable counterstaining. As a control, CD42b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD42b. Human platlets were stained with CD42b antibodies and with a suitable counterstaining. As a control, CD42b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD42b. Human platlets were stained with CD42b antibodies and with a suitable counterstaining. As a control, CD42b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD42b (REA185). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD42b (REA185). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD42b (REA185). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD42b Antibody, anti-human, REAfinity™

Overview

Clone REA185 recognizes CD42b, a 145 kDa single-pass type I membrane protein. Expression of CD42b is found on platelets and megakaryocytes. CD42b, the α chain, together with the β chain (CD42c), forms the heterodimer, glycoprotein Ib (GP Ib). The GP Ib receptor complex further includes association of the α and β subunits with platelet glycoprotein IX and glycoprotein V and this complex serves as the receptor for von Willebrand factor (VWF). The interaction of the GP Ib-IX-V complex to VWF initiates initial platelet adhesion to vascular subendothelium after vascular injury, platelet activation, thrombosis, and hemostasis. Other known ligands for GPIb–IX–V include α-thrombin, clotting factors XI/XIIa, and high-molecularweight kininogen.
Additional information: Clone REA185 displays negligible binding to Fc receptors.

Alternative names

GP1BA, BDPLT1, BDPLT3, BSS, CD42b-alpha, DBPLT3, GP1B, GPIbA, VWDP, gpIbα

Detailed product information

Technical specifications

CloneREA185
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies, Functional-grade antibodies
Specieshuman
Cross-reactivity
chimpanzee (
Pan troglodytes
)
AntigenCD42b
Alternative names of antigenGP1BA, BDPLT1, BDPLT3, BSS, CD42b-alpha, DBPLT3, GP1B, GPIbA, VWDP, gpIbα
Molecular mass of antigen [kDa]67
Distribution of antigenmegakaryocytes, platelets, platelets, megakaryocytes
Entrez Gene ID2811
RRIDAB_2889579, AB_2661343, AB_2658070, AB_2658071, AB_2658074, AB_2658075, AB_2658076, AB_2658077, AB_2658078, AB_2658079, AB_2905009, AB_2905008, AB_2889580

Resources for CD42b Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD42b Antibody, anti-human, REAfinity™

Publications

  1. Shrimpton, C. N. et al. (2002) Localization of the adhesion receptor glycoprotein Ib-IX-V complex to lipid rafts is required for platelet adhesion and activation. J. Exp. Med. 196: 1057
  2. Andrews, R. K. et al. (1997) Molecular mechanisms of platelet adhesion and activation. Int. J. Biochem. Cell Biol. 29(1): 91-105
  3. Andrews, R. K. et al. (2003) Glycoprotein Ib-IX-V. Int. J. Biochem. Cell Biol. 35(8): 1170-1174

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