Clone:
REAL370
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC
Alternative names:
Fc-gamma-RIIB, FCGR3, FcRIII, FcR-10, Fc-gamma RII, FcRII, Ly-17

Extended validation for CD16/CD32 Antibody, anti-mouse,
REAlease
®

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD16/CD32 (REAL370). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD16/CD32 (REAL370). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD16/CD32 (REAL370). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD16/CD32 Antibody, anti-mouse,
REAlease
®

Overview

Clone REAL370 is an antibody fragment derived from the full CD16/CD32 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL370 recognizes a shared epitope common to the human CD16 and CD32 antigens. CD16 and CD32 are also known as FcγIII and FcγII, respectively, and bind to epitopes located in the constant region domain of IgG. CD16 and CD32 are predominantly expressed on cells of hematopoietic lineages, including B cells, monocytes, NK cells, granulocytes, and dendritic cells. They are also found on hematopoietic progenitor cells, where they appear to play a role in T and B cell development.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

Fc-gamma-RIIB, FCGR3, FcRIII, FcR-10, Fc-gamma RII, FcRII, Ly-17

Detailed product information

Technical specifications

CloneREAL370
Clonalitymonoclonal
Isotype controlControl Antibody
Hosthuman cell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD16/CD32
Alternative names of antigenFc-gamma-RIIB, FCGR3, FcRIII, FcR-10, Fc-gamma RII, FcRII, Ly-17
Distribution of antigenB cells, monocytes, NK cells, granulocytes, dendritic cells
RRIDAB_2752129, AB_2752130

Resources for CD16/CD32 Antibody, anti-mouse,
REAlease
®

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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CD16/CD32 Antibody, anti-mouse,
REAlease
®

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