Clone:
REA377
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Fc-gamma-RIIB, FCGR3, FcRIII, FcR-10, Fc-gamma RII, FcRII, Ly-17

Extended validation for CD16/CD32 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA377
Ab93+
2.4G2-
93+
REAL370++
Cells were incubated with an excess of purified unconjugated CD16/CD32 (REA377) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD16/CD32. C57BL/6 mouse splenocytes were stained with CD16/CD32 antibodies and with a suitable counterstaining. As a control, CD16/CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD16/CD32 (REA377). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD16/CD32 (REA377). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD16/CD32 (REA377). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD16/CD32 Antibody, anti-mouse, REAfinity™

Overview

Clone REA377 recognizes a shared epitope common to the human CD16 and CD32 antigens. CD16 and CD32 are also known as FcγIII and FcγII, respectively, and bind to epitopes located in the constant region domain of IgG. CD16 and CD32 are predominantly expressed on cells of hematopoietic lineages, including B cells, monocytes, NK cells, granulocytes, and dendritic cells. They are also found on hematopoietic progenitor cells, where they appear to play a role in T and B cell development.
Additional information: Clone REA377 displays negligible binding to Fc receptors.

Alternative names

Fc-gamma-RIIB, FCGR3, FcRIII, FcR-10, Fc-gamma RII, FcRII, Ly-17

Detailed product information

Technical specifications

CloneREA377
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD16/CD32
Alternative names of antigenFc-gamma-RIIB, FCGR3, FcRIII, FcR-10, Fc-gamma RII, FcRII, Ly-17
Molecular mass of antigen [kDa]26; 33
Distribution of antigenB cells, monocytes, NK cells, granulocytes, dendritic cells
Entrez Gene ID14131, 14130
RRIDAB_2752043, AB_2889472, AB_2655424, AB_2655425, AB_2655428, AB_2655429, AB_2904780, AB_2904779, AB_2752054

Resources for CD16/CD32 Antibody, anti-mouse, REAfinity™

Certificates

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References for CD16/CD32 Antibody, anti-mouse, REAfinity™

Publications

  1. de Andrés, B. et al. (1997) Fc gammaRII (CD32) is linked to apoptotic pathways in murine granulocyte precursors and mature eosinophils. Blood 90(3): 1267-1274
  2. de Andrés, B. et al. (1998) A regulatory role for Fcgamma receptors CD16 and CD32 in the development of murine B cells. Blood 92(8): 2823-2829
  3. Zhang, D. et al. (2014) CD16 inhibition increases host survival in a murine model of severe sepsis. J Surg Res 187(2): 605-609

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