SARS-CoV-2 T Cell Analysis Kit

SARS-CoV-2 T Cell Analysis Kit

Analysis of virus-reactive T cells is essential for understanding fundamental immunological processes in infectious diseases such as COVID-19. However, working with these rare cells can prove challenging to researchers.

Our new SARS-CoV-2 T cell Analysis Kits (PBMC), human ensure efficient stimulation and precise analysis of SARS-CoV-2-reactive T cells.

All-in-one kit for the stimulation and analysis of SARS-CoV-2-reactive T cells**

The kits include: 

  • A SARS-CoV-2 PepTivator® of choice
  • All 8 flow panel antibodies (+1 free position, see table below)
  • A live/dead marker (Viobility™ 405/452 Fixable Dye)
  • Positive stimulation control (CytoStim™), all required buffers and Brefeldin A
  • Optimized intracellular and extracellular staining panels and protocol
  • Compatible with the MACSQuant® Analyzer for highly efficient analysis

SARS-CoV-2 T Cell Analysis Kit variants 

  • SARS-CoV-2 Prot_S  T Cell Analysis Kit (PBMC), human (#130-127-586)
  • SARS-CoV-2 Prot_S1  T Cell Analysis Kit (PBMC), human (#130-127-585)
  • SARS-CoV-2 Prot_S+  T Cell Analysis Kit (PBMC), human (#130-127-584)
  • SARS-CoV-2 Prot_N  T Cell Analysis Kit (PBMC), human (#130-127-583)

Flow panel included in the SARS-CoV-2 T Cell Analysis Kit

MarkerCloneFluorophore 
CD3REA613 APC 
CD4REA623 Viobright-515 
CD8REA734 VioGreen 
CD154 (CD40L) 5C8 APC-Vio770 
IFN-g 45-15 PE 
TNF-a cA2 PE-Vio® 770 
CD14TÜK4VioBlue 
CD20LT20VioBlue 
Live/dead Viobility Fixable Dye 405/452 
Free channel*  PE-Vio® 615 

A panel of 8 fluorophore-conjugated flow antibodies and a live/dead marker (Viobility™ 405/452 Fixable Dye) are included in the kit for precise analysis of SARS-CoV-2-reactive T cells. One channel (PE-Vio®615) is free to allow the addition of one specificity of choice. Additional PE-Vio®615-conjugated specificities may include e.g., IL-2, IL-4, IL-17, CD69, CD137, TIGIT or HLA-DR.
*For VioDye spectra see here

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Analysis of SARS-CoV-2-reactive T cells using  the kit

Human PBMCs from a SARS-CoV-2-reactive donor were incubated for 6 hours with a mix of the SARS-CoV-2 PepTivators Prot_S, Prot_M, and Prot_N or left unstimulated (negative control). Brefeldin A was added after 2 hours. Cells were then stained with the live/dead marker Viobility 405/452 Fixable Dye, fixed and permeabilized afterwards. Subsequently, cells were stained with the flow panel included in the kit (see table). Cells were analyzed using a MACSQuant Analyzer 16. Doublets, debris, dead cells as well as CD14+ and CD20+ cells were excluded. After pregating on CD3 as well as CD4 and CD8, respectively, activation marker and cytokine expression were assessed, e.g. CD154 and TNF-α for CD4+ T cells and TNF-α and IFN-γ for CD8+ T cells.

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Application note
Fast and efficient detection of SARS-CoV-2-reactive T cells via antigen-specific stimulation

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