Pushing the limits of scale - 
Webinar series

Over the past decade, light sheet fluorescence microscopy (LSFM) and tissue clearing technologies have pushed the limits of scale in the field of 3D imaging.  We can now image complete biological systems such as intact organs, tumors, or even whole mice, at cellular resolution in a single imaging volume.  

This online seminar series will provide an inside view into LSFM technologies for high-resolution imaging of large sample volumes.   

Program Overview

Session 1: On Demand Technology Seminar 
Light sheet microscopy for 3D multi-scale imaging of large optically cleared samples 
Presenter: Byron Hartman 
 

Session 2: On Demand Guest Speaker Seminar 
Learning to aggress:  from complex social behavior to whole brain light sheet microscopy
Presenter: Sam Golden 
 

Session 3: On Demand Guest Speaker Seminar 
Mapping the transparent brain with iDISCO+, ClearMap, and light sheet imaging
Presenter: Nicolas Renier 
 

Session 4: On Demand Technology Seminar 
The Ultramicroscope Blaze: Automated 3D imaging on an unprecedented scale
Presenter: Byron Hartman 
 

Session 5: On Demand Technology Seminar  
Ultramicroscope Blaze live instrument demonstration
Presenter: Clare Sengupta 
 

UltraMicroscope Blaze

Abstracts and Registration 

Session 1
Light sheet microscopy for 3D multi-scale imaging of large optically cleared samples
June 30th, 2020 10:00 am PDT
Presented by Byron Hartman Ph.D. Miltenyi Biotec Microscopy Product Manager 

Abstract

This session will introduce the fundamentals of tissue clearing and light sheet imaging and provide an in-depth look at instrument technologies and applications in life science research.  

Over the past decade, the Ultramicroscope instrument series has been essential in the development of new clearing technologies and the progressive expansion of imaging capacity. Now, cited in hundreds of high-impact publications and widely used at most of the top prestigious research institutions around the globe, the Ultramicroscope series of instruments has been improved and expanded to push back still further on the limits of scale.  

Here, we will introduce the Ultramicroscope technology and highlight recent innovations that continue to expand high impact research and discovery in the fields of neuroscience, cancer, developmental biology, and more.  

Session 2
Learning to aggress:  from complex social behavior to whole brain light sheet microscopy
July 9, 2020 11:00 am PDT   Guest Speaker Seminar 
Presented by Sam Golden, Ph.D., Assistant Professor, University of Washington 

Abstract

Maladaptive aggression is an immutable force that contributes to the suffering and death of millions of people around the world annually, as well as presents significant treatment challenges to physicians and care-takers. While aggression can be highly rewarding and pursued despite adverse consequences, as well as sought after lengthy abstinence, maladaptive aggression is not generally viewed as a component of neuropsychiatric disease states.

Consequently, little progress has been made in the treatment of pathological aggression. What drives an individual to voluntarily seek out an aggressive interaction? How does aggression shift from an evolutionally conserved, and highly adaptive process, to a maladaptive and pathological state?  As such, aggression is an ethologically complex behavior with equally complex underlying mechanisms.

Here, Dr. Sam Golden will present data on one form, appetitive (rewarding) aggression, and the behavioral, cellular and system-level mechanisms guiding this behavior. He will briefly present how appetitive aggression is behaviorally modeled in mice, and extend the concept of aggression motivation to compulsive aggression seeking and relapse.  He will then highlight recent advances in computer vision and machine learning for automated predictive classification of aggressive behavior, the role of specific cell-types in controlling aggression reward, and close with preliminary data on the whole brain functional connectome of aggression reward using light sheet fluorescent microscopy (LSFM). Together, these approaches bridge the analysis of complex social behavior with whole brain, single-cell resolution, connectomics. 

Session 3
Mapping the transparent brain with iDISCO+, ClearMap, and light sheet imaging 
July 21st, 2020  8 am PDT   Guest Speaker Seminar 
Presented by Nicolas Renier PhD, Group Leader, ICM - Brain and Spine Institute, Paris, France

Abstract

The adult brain is composed of densely interwoven neuronal and vascular networks that are essential for proper function.  Understanding the structural organization and dynamic changes of these networks is highly challenging  because of their complex 3D topology.  

Dr. Nicolas Renier and his colleagues developed the iDISCO+ and ClearMap technologies to facilitate the study of the structure and cellular networks in the intact adult brain with automated quantitative methods.  Together with light sheet fluorescence microscopy (LSFM), these technologies enable whole brain 3D imaging and detailed quantitative mapping.  iDISCO+ is a complete sample preparation method that enables whole-mount immunolabeling and high-performance optical clearing of adult brain, or any organ.  ClearMap is an open-source software program for the unsupervised mapping of neuronal activity, neuronal projections, and the vascular networks of the brain.  These technologies form a pipeline for high speed acquisition of brain structure and activity at cellular resolution.  

The workflow consists of sample preparation with immunostaining and tissue clearing, then imaging the whole brain with LSFM for expression profiling , and finally automated mapping and analysis.  Dr. Renier will present an overview of these tools and discuss how his team is using them to characterize the plasticity of adult brain networks.

Session 4
The Ultramicroscope Blaze: Automated 3D imaging on an unprecedented scale 
August 4th, 2020 10:00 am PDT   Technology Seminar 
Presented by Byron Hartman Ph.D. Miltenyi Biotec Microscopy Product Manager 

Abstract

This webinar will introduce the technology of the brand new Ultramicroscope Blaze, a next-generation light sheet imaging system that brings together more than a decade of LSFM development and collaboration. 

The Blaze is a true multi-scale instrument that can capture cellular detail at submicron resolution and has the capacity to image very large samples such as a whole, optically cleared, mouse body.  This system is ideal for high-volume, high-throughput applications for imaging samples such as: all mouse organs, whole mice, tumors from xenografts or human patients, and small human organs.  

Join us for this exciting first look at the new Ultramicroscope Blaze.

Session 5
Ultramicroscope Blaze live instrument demonstration
August 11, 2020 10:00 am PDT  Technology Seminar   
Presented by Clare Sengupta, Ph.D. Miltenyi Biotec Field Applications Scientist 

Abstract

This live demo will introduce you directly to the Ultramicroscope Blaze, a fully-automated, multi-scale, light sheet imaging system for high throughput volumetric imaging of large biological samples at cellular resolution.  

Dr. Sengupta will demonstrate the ease-of-use and performance of the instrument and take you through the experience of operating it to acquire data.  This will include a system overview and highlights of the adjustable light sheet generation pathway, precision detection optics, motorized objective turret and magnification changers, automated tiling and batch acquisition, and much more. 

Background information on light sheet microscopy