Meet the author – publications using UltraMicroscope technology

Meet the author – publications using UltraMicroscope technology

Scientists describe how UltraMicroscope 3D light sheet imaging helped them advance their research

Conducting a research study and ultimately publishing the results can be a rocky road: from designing the project and overcoming emerging challenges to enjoying what you’ve accomplished. During the ongoing pandemic we had the opportunity to speak with authors of recent papers covering a wide range of research fields – from cardiovascular research and immuno-oncology through to virology. In these short videos, recorded during an online call, the authors share their experiences working on their projects and describe how they reached their goals. They all used 3D light sheet microscopy and UltraMicroscope technology to support their hypotheses and advance their research.

The evolution of 3D vision – understanding the cellular basis

Video

Robin Vigouroux, Ph.D.

Institut de la vision, Sorbonne Université, Paris, France

Robin Vigouroux speaks on his project investigating the evolution of 3D vision. A sophisticated tissue clearing method (EyeDISCO) allowed him and his coauthors to image whole bilateral visual projections from the eye to the brain by 3D light sheet microscopy in various species. 

Read more about this study and have a look at the data:

Vigouroux, R. et al. (2021) Bilateral visual projections exist in non-teleost bony fish and predate the emergence of tetrapods. Science 372: 150–156. DOI: 10.1126/science.abe7790.

Pancreatic cancer models – evidence for direct interaction between myeloid-derived suppressor cells and regulatory T cells

Video

Carole Siret, Ph.D.

Centre d’Immunologie de Marseille-Luminy, Marseille, France

Carole Siret delineates her project deciphering the cross-talk between immunosuppressive cells in mouse pancreatic cancer. Using the UltraMicroscope light sheet imaging system, she and her colleagues were able to visualize myeloid-derived suppressor cells, regulatory T cells, and the vasculature in 3D.

Read more about this study and have a look at the data:

Siret, C. et al. (2020) Deciphering the crosstalk between myeloid-derived suppressor cells and regulatory T cells in pancreatic ductal adenocarcinoma. Front. Immunol. 10: 3070.  DOI: 10.3389/fimmu.2019.03070.
 

NK cells protect the brain – how the UltraMicroscope advances stroke research

Video

Serge Van De Pavert, Ph.D.

Centre d’Immunologie de Marseille-Luminy, Marseille, France

Serge Van De Pavert describes a project elucidating the role of innate lymphoid cells (ILCs) in stroke. Using 3D fluorescence light sheet microscopy, he and his colleagues showed that various ILCs migrated into ischemic lesions of a mouse model.

Read more about this study and have a look at the data:

Wang, S. et al. (2021) Brain endothelial CXCL12 attracts protective natural killer cells during ischemic stroke. bioRxiv: preprint. DOI: 10.1101/2021.02.18.431426.
 

The UltraMicroscope sheds light on the mechanism of viral pathogenesis

Video

Dr. Luca Zaeck

Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany

Luca Zaeck talks about how the UltraMicroscope enabled him and his colleagues to analyze abundance, localization, and distribution of SARS-CoV-2 in the 3D morphological context of intact respiratory tissue of the ferret model. 

Read more about this study and have a look at the data:

Zaeck, L.M. et al. (2021) Light sheet microscopy-assisted 3D analysis of SARS-CoV-2 infection in the respiratory tract of the ferret model. Viruses 13: 529. DOI: 10.3390/v13030529. 

Insights into heart damage and response processes caused by infarction

Video

Sebastian Korste, Ph.D.

Dept. of Cardiology and Vascular Medicine, University Hospital Essen, Essen, Germany

Listen to Sebastian Korste as he talks about a study in which he and his colleagues measured several parameters defining the damage extent and immune response in the heart after infarction. 3D light sheet imaging data generated with the UltraMicroscope afforded them a full view of the heart in a mouse model. 

Read more about this study and have a look at the data:

Merz, S.F., Korste, S., Bornemann, L. et al. (2019) Contemporaneous 3D characterization of acute and chronic myocardial I/R injury and response. Nat. Commun. 10: 2312. DOI: 10.1038/s41467-019-10338-2.
 

Discovering non-conventional macrophages in the brain

Video

Serge Van De Pavert, Ph.D.

Centre d’Immunologie de Marseille-Luminy, Marseille, France

In this video, Serge Van De Pavert explains how he and his colleagues discovered a non-conventional macrophage population in the brain that could play a role in different kinds of brain diseases. They used an UltraMicroscope light sheet imaging system to visualize these cells in whole mouse brains. 

Read more about this study and have a look at the data:

Siret, C. et al. (2021) Identification and characterization of a non-conventional CD45 negative perivascular macrophage population within the mouse brain. Research Square: preprint. DOI: 10.21203/rs.3.rs-479980.

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