Save 50% on your first bottle of serum-free astrocyte culture medium

Are you looking for a serum-free medium that supports excellent astrocyte growth in vitro? Or do you want to culture astrocytes from adult mouse brain? We have just the right thing for you!

Try our new serum-free AstroMACS Medium and start taking advantage of:

  • Superior neonatal astrocyte growth even at very low seeding densities
  • Enhanced adult astrocyte culture with the new AstroMACS Separation Buffer, which removes dead cells during cell separation and provides a healthy culture environment
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Superior astrocyte growth even at low seeding densities

Neonatal astrocytes were isolated from P4 mice using the Neural Tissue Dissociation Kit (P) and the Anti-ACSA-2 MicroBead Kit. Cells were cultured in MACS® Neuro Medium and MACS NeuroBrew®-21 (top) or in AstroMACS Medium (bottom) at different densities in a 24-well imaging plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (yellow) and DAPI (blue) and dead and viable astrocytes were analyzed with the Cytation™ Cell Imaging Multi-Mode Reader. (A) Microscope images of one representative well. (B) Quantitative data of 4 replicates.

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AstroMACS Separation Buffer removes dead cells and enhances adult astrocyte culture

Adult astrocytes were isolated from 8-week-old mouse brains using the Adult Brain Dissociation Kit and the Anti-ACSA-2 MicroBead Kit in combination with (A) or without (B) the AstroMACS Separation Buffer. Cells were then cultivated in AstroMACS Medium at a density of 100,000 cells/well in a 24-well Imaging Plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).

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Preservation of healthy astrocytes for reliable downstream experiments

Neonatal astrocytes were isolated from P4 mice using the Neural Tissue Dissociation Kit (P) and the Anti-ACSA-2 MicroBead Kit, and cultured in AstroMACS Medium at a density of 10,000 cells/well in a 24-well imaging plate for 7 days. Cells were then fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).

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Please use the promo code AstroMACS_2018 when you place an order to receive your 50% discount on your first bottle of AstroMACS Medium and/or AstroMACS Separation Buffer

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