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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Whether you perform flow cytometry, flow sorting, or microscopy, our portfolio of fluorophores – covering wavelengths from 400 nm to 800 nm – enables you to do comprehensive multicolor analysis.
Our Vio Dye family of fluorophores includes both single fluorochromes and tandem dyes. In addition various traditional fluorophores are available to allow for fluorescence analysis over a wide range of the visual spectrum.
Fluorescent dye | Excitation (nm) | Exmax (nm) | Emmax (nm) |
---|---|---|---|
Violet laser fluorochromes | |||
VioBlue® | 405 | 400 | 452 |
VioGreenTM | 405 | 388 | 520 |
Vio® Bright V423 | 405 | 399 | 420 |
Blue laser tandem dyes | |||
Vio® B515 | 488 | 488 | 514 |
Vio® Bright B515 | 488 | 488 | 514 |
Vio® Bright FITC | 488 | 496 | 522 |
FITC | 488 | 495 | 520 |
Blue and yellow-green laser dyes | |||
PE-Vio® 615 | 488 or 561 | 565 | 619 |
PE-Vio® 770 | 488 or 561 | 565 | 775 |
PE | 488 or 561 | 565 | 578 |
PerCP-Vio® 700 | 488 | 482 | 704 |
PerCP | 488 | 482 | 675 |
Red laser fluorochromes | |||
Vio® R667 | 561 or 635 or 640 | 645 | 668 |
Vio® Bright R667 | 561 or 635 or 640 | 645 | 668 |
Vio® R720 | 640 | 695 | 720 |
Vio® Bright R720 | 640 | 695 | 720 |
APC-Vio® 770 | 561 or 635 or 640 | 652 | 775 |
APC | 561 or 635 | 652 | 660 |
To help you plan your experiment properly, the table below summarizes important characteristics of all Vio Dye fluorophores that are available for Miltenyi Biotec antibodies.
Characteristics of fluorescent dyes
Fluorophore | Brightness | Fixation stability | Photostability | MACSQuant Analyzer Detection filter (nm) | Microscopy |
---|---|---|---|---|---|
Violet laser fluorescent dyes | |||||
VioBlue | 3 | PFA, ethanol, methanol, and acetone | Medium | 450/50 | Not tested |
VioGreen | 1 | PFA, ethanol, methanol, and acetone | High | 525/50 | Not tested |
Vio Bright V423 | 5 | PFA | High | 450/50 | Not tested |
Blue laser fluorochromes | |||||
Vio B515 | 4 | PFA, ethanol, methanol, and acetone | Very high | 525/50 | Recommended |
Vio Bright B515 | 5 | PFA, ethanol, methanol, and acetone | Medium | 525/50 | Suitable |
Vio Bright FITC | 4 | PFA, ethanol, methanol, and acetone | Low | 525/50 | Suitable |
Blue and yellow-green laser tandem dyes | |||||
PE-Vio 615 | 5 | PFA | Not tested | 655-730 or 615/20 | Suitable |
PE-Vio 770 | 4 | PFA | Not tested | 750 LP or 667/30 | Not tested |
PerCP-Vio 700 | 3 | PFA | Not tested | 655-730 or 615/20 | |
Red laser fluorescent dyes | |||||
APC-Vio 770 | 3 | PFA | Not tested | 750 LP or 725/40 | Not tested |
Vio R667 | 5 | PFA, ethanol, methanol, and acetone | Very high | 655-730 or 661/20 or 667/30 | Recommended |
Vio Bright R667 | 5+ | PFA, ethanol, methanol, and acetone | Medium | 655-730 or 661/20 or 667/30 | Suitable |
Vio R720 | 3 | PFA | High | 725/40 | Not tested |
Vio Bright R720 | 4 | PFA | High | 725/40 | Not tested |
Vio Bright V423 is Miltenyi Biotec’s brightest dye, excited at 405 nm. In addition to high brightness, this dye shows a significantly improved signal-to-noise ratio, minimal intra-laser compensation, and a superior detection of rare antigens.
Vio Bright V423 is a synthetic dye which is 5× brighter than VioBlue Dye, making it an excellent choice for multiparameter cell analysis.
Vio Bright V423 at a glance:
Excitation and emission maxima of fluorochromes related to Vio Bright V423.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
Vio Bright V423 | 399 | 420 |
Brilliant Violet 421 (BD) | 408 | 425 |
Brilliant Violet 421 (Biolegend) | 408 | 424 |
Super Bright 436 | 415 | 434 |
Designed to maximize the potential of a flow cytometer’s violet laser, the VioBlue Dye shows superior performance compared to many other fluorophores excited at 405 nm, including significant advances in brightness, signal-to-noise ratios, and intralaser compensation requirements. In addition, VioBlue exhibits minimal photo-induced degradation, and can consequently be used for many different applications, such as fluorescence microscopy.
VioBlue Dye at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Excitation and emission maxima of fluorochromes related to VioBlue.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
VioBlue | 400 | 455 |
Pacific Blue | 405 | 455 |
Cascade Blue | (375); 401 | 423 |
Alexa Fluor 405 | 405 | 421 |
eFluor 405 | 405 | 450 |
BD Horizon V450 | 404 | 448 |
Designed to maximize the potential of a flow cytometer’s violet laser, the VioGreen Dye shows superior performance compared with many other fluorophores excited at 405 nm, including significant advances in brightness, signal-to-noise ratios, and intralaser compensation requirements. In addition, VioGreen exhibits minimal levels of photo-induced degradation, and can consequently be used for many different applications, such as fluorescence microscopy.
VioGreen Dye at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Excitation and emission maxima of fluorochromes related to VioGreen.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
VioGreen | 388 | 520 |
Pacific Orange | 400 | 551 |
Krome Orange | 398 | 528 |
AmCyan | 458 | 489 |
HorizonV500 | 415 | 500 |
Vio Bright B515 and Vio B515 are extremely bright dyes for the B1/FITC-channel. The proprietary Vio Bright multimerization technology results in a 4-fold increase in brightness over the traditional FITC dye. With a 25% reduced spill-over into the B2/PE-channel and high photo stability, these dyes are excellent choices for immunofluorescence microscopy applications.
Vio Bright B515 at a glance:
Vio B515 at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Vio Bright FITC is a blue laser–excited (488 nm) revolutionary dye, which allows binding of an increased number of FITC molecules per antibody, as compared to conventional FITC conjugation. With brightness similar to PE, Vio Bright FITC expands the dimensions of multicolor flow analysis. In addition, it provides a bright alternative for confident detection of rare cells, as well as dim and uncharacterized markers.
VioBright FITC at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Excitation and emission peaks of fluorochromes compared to Vio Bright B515, Vio B515, and Vio Bright FITC.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
Vio Bright B515 | 488 | 514 |
Vio B515 | 488 | 514 |
VioBright FITC | 496 | 522 |
FITC | 495 | 520 |
Alexa Fluor 488 | 495 | 519 |
BD Horizon Brilliant Blue 515 | 490 | 515 |
The PerCP-Vio 700 Dye is a tandem conjugate that combines the peridinin chlorophyll protein (PerCP) and the new Vio 700 Dye to emit a strong fluorescence at 655–730 nm upon blue laser excitation at 488 nm. This dye is suited perfectly for the B3 channel of the MACSQuant Analyzer.
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Excitation and emission maximums of fluorochromes comparable to PerCP-Vio 700.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
PerCP | 482 | 675 |
PerCP-Vio 700 | 482 | 704 |
PerCP-Cy5.5 | 490 | 695 |
The PE-Vio 770 Dye is a tandem conjugate, like PE-Cy™7, that exploits the principle of fluorescence-resonance-energy-transfer (FRET) allowing for large Stokes shifts between the absorbed energy of a fluorescence donor (PE) and the emission wavelength of a suitable acceptor (Vio 770) dye.
PE-Vio 770 Dye at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Excitation and emission maximums of fluorochromes comparable to PE-Vio 770.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
PE-Vio 770 | 565 | 775 |
PE-Cy7 | 496 | 774 |
PE-Alexa Fluor 750 | 496 | 775 |
PE-Vio 615 is a tandem dye with PE as the donor dye and Vio 615 as the acceptor dye. This tandem dye is optimized for efficient donor-to-acceptor dye energy transfer, high fluorescent intensity, and low spillover into the donor dye detection channel. Designed to be a superior alternative to ECD, PE-Texas Red®, PE-efluor® 610, PE-CF594 and PE/Dazzle™ 594, this Vio Dye expands the options for flexible multicolor panel design and provides a bright dye for confident detection of dim and rare markers.
PE-Vio 615 at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Excitation and emission maximums of fluorochromes comparable to PE-Vio 615.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
PE-Vio 615 | 565 | 619 |
ECD, PE-Texas Red | 565 | 613 |
PE-efluor 610 | 565 | 606 |
PE-CF594 | 565 | 614 |
PE/Dazzle 594 | 566 | 612 |
The APC-Vio 770 Dye is a tandem conjugate, like APC-Cy™7 or APC-H7, that exploits the principle of fluorescence-resonance-energy transfer (FRET) allowing for large Stokes shifts between the absorbed energy of a fluorescence donor (APC) and the emission wavelength of a suitable acceptor (Vio 770) dye.
APC-Vio 770 Dye at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Excitation and emission maxima of fluorochromes related to APC-Vio 770.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
APC-Vio 770 | 625 | 775 |
APC-Cy7 | 650 | 774 |
APC-Alexa Fluor | 750 | 775 |
Vio Bright R667 and Vio R667 are extremely bright dyes for the R1/APC-channel. The proprietary Vio Bright multimerization technology results in a two-fold increase in brightness over the traditional APC dye.
Vio Bright R667 at a glance:
Vio R667 at a glance:
For detailed information including stability, spillover, and brightness data, please visit the MACS Handbook.
Excitation and emission maxima of fluorochromes related to APC.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
Vio Bright R667 | 645 | 668 |
Vio R667 | 645 | 668 |
APC | 650 | 660 |
Vio Bright R720 and Vio R720 are organic dyes that can be detected in Alexa Fluor 700 channel (R2 channel of MACSQuant® Analyzer 16). The proprietary multimerization technology underlying the Vio Bright R720 dye typically allows for a more than 2-fold increase in brightness over the non-multimerized Vio R720. This makes Vio Bright R720 an excellent choice for multiparameter cell analysis.
Vio Bright R720 at a glance:
Vio R720 at a glance:
Excitation and emission maxima of fluorochromes related to APC.
Fluorochrome | Excitation max. (nm) | Emission max. (nm) |
---|---|---|
Vio Bright R720 | 695 | 720 |
Vio R720 | 695 | 720 |
Alexa Fluor 700 (BD) | 697 | 722 |
APC-R700 | 653 | 713 |
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