The IL-4 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as enrichment of human IL-4-secreting cells.

Data and images for IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-4 using the IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 24 IL-4 secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-4 secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment. There seemed to be no difference between stimulated and unstimulated sample before enrichment.
Stimulated sample
Before enrichment
After enrichment
View details

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-4 using the IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 24 IL-4 secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-4 secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment. There seemed to be no difference between stimulated and unstimulated sample before enrichment.
View details

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-4 using the IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 24 IL-4 secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-4 secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment. There seemed to be no difference between stimulated and unstimulated sample before enrichment.
Unstimulated control
Before enrichment
After enrichment
View details

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-4 using the IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 24 IL-4 secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-4 secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment. There seemed to be no difference between stimulated and unstimulated sample before enrichment.
View details

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-4 using the IL-4 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 24 IL-4 secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-4 secreting CD4
+
T cells were detected per 10
6
CD4
+
T cells after enrichment. There seemed to be no difference between stimulated and unstimulated sample before enrichment.

Specifications for IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The IL-4 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as enrichment of human IL-4-secreting cells.

Detailed product information

Background information

Interleukin-4 (IL-4) is predominantly secreted by activated CD4
+
memory and effector T
H
2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The T2 types of immune mechanisms can also be involved in immunologic disorders such as IgE-mediated allergy.

Downstream applications

IL-4 Secretion Assay was used to isolate and analyze Tetanus Toxoid-specific
1
, allergen-specific
2,5
, and autoreactive
7
T cells from PBMCs, as well as tumor-specific cells from T cell lines
3
. The IL-4 Secretion Assay was also used for isolation of IL-4-secreting human NK cells
4
, and for detection of IL-4-secreting NKT cells
6
.
IL-4-secreting CD8
+
T cells were isolated in combination with CD8 MultiSort MicroBeads.
8

Columns

MS, LS, or autoMACS
®
Columns.

Resources for IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

References for IL-4 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Publications

  1. Brosterhus, H. et al. (1999) Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion. Eur. J. Immunol. 29: 4053-4059
  2. Akdis et al. (2000) MACS&more 4: 9-10
  3. Oelke, M. et al. (2000)
    Functional characterization of CD8
    +
    antigen-specific cytotoxic T lymphocytes after enrichment based on cytokine secretion: comparison with the MHC-tetramer technology.
    Scand. J. Immunol. 52: 544-549
  4. Deniz, G. et al. (2002) Human NK1 and NK2 subsets determined by purification of IFN-gamma-secreting and IFN-gamma-nonsecreting NK cells. Eur. J. Immunol. 32: 879-884
  5. Akdis, M. et al. (2004) Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. J. Exp. Med. 199: 1567-1575
  6. Araki, M. et al. (2002)
    Tʜ2 bias of CD4
    +
    NKT cells derived from multiple sclerosis in remission.
    Int. Immunol. 15: 279-288
  7. Veldman, C. et al. (2003) Dichotomy of autoreactive Th1 and Th2 cell responses to desmoglein 3 in patients with pemphigus vulgaris (PV) and healthy carriers of PV-associated HLA class II alleles. J. Immunol. 170: 635-642
  8. Schwaiger, S. et al. (2003)
    IL-4-producing CD8
    +
    T cells with a CD62L
    ++(bright)
    phenotype accumulate in a subgroup of older adults and are associated with the maintenance of intact humoral immunity in old age.
    J. Immunol. 170: 613-619

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