Clone:
REA437
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
OX-8, Lyt2, Ly-2

Extended validation for CD8a Antibody, anti-rat, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA437
OX-8-
G28++
341-
REA222-
Cells were incubated with an excess of purified unconjugated CD8a (REA437) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD8a. Winstar rat splenocytes were stained with CD8a antibodies and with a suitable counterstaining. As a control, CD8a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD8a. Winstar rat splenocytes were stained with CD8a antibodies and with a suitable counterstaining. As a control, CD8a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD8a. Winstar rat splenocytes were stained with CD8a antibodies and with a suitable counterstaining. As a control, CD8a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD8a. Winstar rat splenocytes were stained with CD8a antibodies and with a suitable counterstaining. As a control, CD8a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD8a. Winstar rat splenocytes were stained with CD8a antibodies and with a suitable counterstaining. As a control, CD8a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD8a (REA437). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD8a (REA437). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD8a (REA437). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD8a Antibody, anti-rat, REAfinity™

Overview

Clone REA437 recognizes the rat CD8a antigen, a single-pass type I membrane protein, which is also known as OX-8. CD8 acts as a co-receptor for the T cell receptor and recognizes antigen displayed by an antigen presenting cell in the context of class I MHC molecules. CD8a is expressed as a heterodimer with CD8b on T cell receptor (TCR) α/β and TCR γ/δ expressing, cytotoxic T cells, and on most thymocytes. As a homodimer of two CD8a chains the CD8 antigen is expressed on most NK cells, a major fraction of intestinal intraepithelial lymphocytes, some activated CD4
+
CD8
+
T cells and CD8
+
T cells from athymic rats. CD8a is also expressed by a T cell subset with regulatory functions.
Additional information: Clone REA437 displays negligible binding to Fc receptors.

Alternative names

OX-8, Lyt2, Ly-2

Detailed product information

Technical specifications

CloneREA437
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesrat
AntigenCD8a
Alternative names of antigenOX-8, Lyt2, Ly-2
Molecular mass of antigen [kDa]23
Distribution of antigenNK cells, T cells, thymocytes, T helper cells
Entrez Gene ID24930
RRIDAB_2819701, AB_2659473, AB_2659474, AB_2659475, AB_2659476, AB_2659477, AB_2659478, AB_2659479, AB_2659480, AB_2659481, AB_2659482, AB_2659485, AB_2659486, AB_2659487, AB_2659488, AB_2751789

Resources for CD8a Antibody, anti-rat, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD8a Antibody, anti-rat, REAfinity™

Publications

  1. Johnson, P. et al. (1985) Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes. EMBO J. 4(10): 2539-2545
  2. Torres-Nagel, N. et al. (1992) Differential thymus dependence of rat CD8 isoform expression. Eur. J. Immunol. 22(11): 2841-2848
  3. Strup-Perrot, C. et al. (2005) Expression of matrix metalloproteinases and tissue inhibitor metalloproteinases increases in X-irradiated rat ileum despite the disappearance of CD8a T cells. World J Gastroenterol. 11(40): 6312-6321

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