Clone:
REA905
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Intercellular adhesion molecule 3, CDw50, ICAM-R, ICAM3

Extended validation for CD50 (ICAM-3) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD50 (ICAM-3) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD50 (ICAM-3)-PE (REA905, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD50 (ICAM-3) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD50 (ICAM-3)-PE (REA905, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD50 (ICAM-3) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD50 (ICAM-3)-PE (REA905, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD50 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD50-PE, clone (REA905). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD50 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD50-PE, clone (REA905). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD50 (ICAM-3) Antibody, anti-human, REAfinity™

Overview

Clone REA905 recognizes the human CD50 antigen, a type I transmembrane glycoprotein also known as intercellular adhesion molecule 3 (ICAM-3). As a member of the immunoglobulin superfamily, CD50 is closely related to ICAM-1 and consists of five immunoglobulin domains. It is expressed by leukocytes and is a ligand for CD11a (leukocyte adhesion LFA-1 protein). CD50 is also associated with the amino-terminal domain of ERM proteins and moesin and plays an essential role in primary immune responses.
Additional information: Clone REA905 displays negligible binding to Fc receptors.

Alternative names

Intercellular adhesion molecule 3, CDw50, ICAM-R, ICAM3

Detailed product information

Technical specifications

CloneREA905
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD50 (ICAM-3)
Alternative names of antigenIntercellular adhesion molecule 3, CDw50, ICAM-R, ICAM3
Molecular mass of antigen [kDa]56
Distribution of antigenleukocytes
Entrez Gene ID3385
RRIDAB_2726924, AB_2726983, AB_2726925, AB_2726984, AB_2726926, AB_2726988, AB_2726930, AB_2726989, AB_2726931, AB_2726985, AB_2726927, AB_2726986, AB_2726928, AB_2726987, AB_2726929, AB_2726981, AB_2726923, AB_2726982

Resources for CD50 (ICAM-3) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD50 (ICAM-3) Antibody, anti-human, REAfinity™

Publications

  1. de Fougerolles, A. R. et al. (1992) Intercellular adhesion molecule 3, a third adhesion counter-receptor for lymphocyte function-associated molecule 1 on resting lymphocytes. J. Exp. Med. 175(1): 185-190
  2. Serrador, J. M. et al. (2002) A novel serine-rich motif in the intercellular adhesion molecule 3 is critical for its ezrin/radixin/moesin-directed subcellular targeting. J. Biol. Chem. 277(12): 10400-10409

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