Clone:
REA191
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
TREM1

Extended validation for CD354 (TREM-1) Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA191
174031++
L5-B8.2A12.3A12++
TR3MBL1-
Cells were incubated with an excess of purified unconjugated CD354 (TREM-1) (REA191) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD354 (TREM-1). Balb/c splenocytes were stained with CD354 (TREM-1) antibodies and with a suitable counterstaining. As a control, CD354 (TREM-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cells were pregated on CD8a negative cells. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD354 (TREM-1). Balb/c splenocytes were stained with CD354 (TREM-1) antibodies and with a suitable counterstaining. As a control, CD354 (TREM-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cells were pregated on CD8a negative cells. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD354 (TREM-1). Balb/c splenocytes were stained with CD354 (TREM-1) antibodies and with a suitable counterstaining. As a control, CD354 (TREM-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cells were pregated on CD8a negative cells. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD354 (TREM-1). Balb/c splenocytes were stained with CD354 (TREM-1) antibodies and with a suitable counterstaining. As a control, CD354 (TREM-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cells were pregated on CD8a negative cells. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD354 (TREM-1) (REA191). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD354 (TREM-1) (REA191). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD354 (TREM-1) (REA191). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD354 (TREM-1) Antibody, anti-mouse, REAfinity™

Overview

Clone REA191 recognizes murine CD354 (TREM-1), a 30 KDa member of TREM (triggering receptors expressed on myeloid cells) family of cell surface receptors. TREM receptors are immunoglobulin 'superfamily' members and contain a single variable-type immunoglobulin domain. Murine TREM cluster is located on chromosome 17C. Expression of TREM-1 is found on cell surface of myeloid cells such as neutrophils, monocytes, dendritic cells and macrophages. TREM-1 signals via ITAM-containing adaptor DAP12 and serves as an amplifier of cell activation in response to triggers such as LPS . TREM-1 activated cellular signalling molecules and pathways lead to generation of pro-inflammatory response such as expression and secretion of chemokines and cytokines including MCP-1, MIP-1α, IL-6,-8 TNF. TREM-1 acts in a concerted manner with other signalling receptors as TLRs and Nod-like receptors, leading to an amplification of initial response. Expression of TREM-1 itself is upregulated in response to presence of LPS, microbial products and cytokines released during activation response. A soluble form of TREM-1 (sTREM-1) is also detected in serum of mouse infection, sepsis and granulomatous disease models.
Additional Information: Clone REA191 displays negligible binding to Fc receptors.

Alternative names

TREM1

Detailed product information

Technical specifications

CloneREA191
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD354 (TREM-1)
Alternative names of antigenTREM1
Distribution of antigendendritic cells, macrophages, neutrophils
RRIDAB_2657697, AB_2657698, AB_2657699, AB_2657700, AB_2657701, AB_2657702, AB_2657703, AB_2657696

Resources for CD354 (TREM-1) Antibody, anti-mouse, REAfinity™

Certificates

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References for CD354 (TREM-1) Antibody, anti-mouse, REAfinity™

Publications

  1. Arts, R. J. W. et al. (2013) TREM-1: intracellular signaling pathways and interaction with pattern recognition receptors. J. Leukoc. Biol. 93: 209-215
  2. Wu, M. et al. (2011)
    TREM-1 amplifies corneal inflammation after
    Pseudomonas aeruginosa
    infection by modulating Toll-like receptor signaling and Tʜ1/Tʜ2-Type immune responses.
    Infect. Immun. 79: 2709 -2716
  3. Ferat-Osorio, E. et al. (2008) The increased expression of TREM-1 on monocytes is associated with infectious and noninfectious inflammatory processes. J. Surg. Res. 150(1): 110-117

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