Clone:
HI149
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC
Alternative names:
HTA1, CD1, FCB6, R4, T6, Leu-6, CD1a

Extended validation for CD1a Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with HI149
REA736+
NA1/34-HLK-
SK9-
Cells were incubated with an excess of purified unconjugated CD1a (HI149) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD1a (HI149). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD1a (HI149). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD1a (HI149). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD1a Antibody, anti-human

Overview

Clone HI149 reacts with human CD1a, a 40kDa type I membrane glycoprotein also known as T6 which is a member of the immunglobulin superfamily. It shares functional and structural similarities with MHC I class molecules and is associated with β2-microglobulin. CD1a plays a role in antigen presentation. It is expressed on Langerhans cells, dendritic cells, and cortical thymocytes.

Alternative names

HTA1, CD1, FCB6, R4, T6, Leu-6, CD1a

Detailed product information

Technical specifications

CloneHI149
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD1a
Alternative names of antigenHTA1, CD1, FCB6, R4, T6, Leu-6, CD1a
Molecular mass of antigen [kDa]35
Distribution of antigenT cells, dendritic cells, Langerhans cells, macrophages, monocytes, B cells, thymocytes
Entrez Gene ID909
RRIDAB_2857677, AB_2656019, AB_2656021, AB_2656022, AB_2656023, AB_2656024, AB_2656025, AB_2656026, AB_2656027, AB_2857689

Resources for CD1a Antibody, anti-human

Certificates

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References for CD1a Antibody, anti-human

Publications

  1. Blumberg, R. S. et al. (1995) Structure and function of the CD1 family of MHC-like cell surface proteins. Immunol. Rev. 147: 5-29
  2. Hanau, D. et al. (1990) Possible mechanism of action of CD1a antigens. J. Invest. Dermatol. 95(5): 503-505
  3. Calabi, F. et al. (1991) The CD1 system. Tissue Antigens 37(1): 1-9

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