Clone:
REA649
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
CXCR4, Fusin, LESTR, FB22, HM89, LCR1, LAP-3, NPYRL

Extended validation for CD184 (CXCR4) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA649
12G5++
Cells were incubated with an excess of purified unconjugated CD184 (CXCR4) (REA649) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD184 (CXCR4) (REA649). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD184 (CXCR4) (REA649). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD184 (CXCR4) (REA649). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD184 (CXCR4) Antibody, anti-human, REAfinity™

Overview

Clone REA649 recognizes the human CD184 antigen, a multi-pass membrane protein, also known as C-X-C chemokine receptor type 4 (CXCR4), leukocyte-derived seven transmembrane domain receptor (LESTR), or fusin. CD184 is ubiquitously expressed on blood and tissue cells. It mediates chemotaxis in mature and progenitor blood cells and is important for B lymphopoiesis, myelopoiesis, and cardiogenesis. CD184 exclusively interacts with the endogenous ligand CXCL12. Binding of CXCL12 transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Although CXCL12 is the only known chemokine that binds CD184, recent studies suggest that extracellular ubiquitin also acts as an immune modulator through CD184-mediated signaling. CD184 is a coreceptor for HIV entry by promoting Env-mediated fusion of the virus.
Additional information: Clone REA649 displays negligible binding to Fc receptors.

Alternative names

CXCR4, Fusin, LESTR, FB22, HM89, LCR1, LAP-3, NPYRL

Detailed product information

Technical specifications

CloneREA649
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
,
rhesus monkey (
Macaca mulatta
)
,
african green monkey (
Chlorocebus aethiops
)
, baboon,
chimpanzee (
Pan troglodytes
)
,
sooty mangabey (
Cercocebus atys
)
AntigenCD184 (CXCR4)
Alternative names of antigenCXCR4, Fusin, LESTR, FB22, HM89, LCR1, LAP-3, NPYRL
Molecular mass of antigen [kDa]40
Distribution of antigenB cells, dendritic cells, endothelial cells, epithelial cells, granulocytes, megakaryocytes, monocytes, neurons, platelets, T cells, leukocytes, lymphocytes, hematopoietic stem and progenitor cells, mesenchymal stem cells, pancreatic carcinoma cells, thymocytes, brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen
Entrez Gene ID7852
RRIDAB_2734060, AB_2733779, AB_2733780, AB_2727647, AB_2727587, AB_2752001, AB_2751970, AB_2752192, AB_2752173, AB_2857538, AB_2857534, AB_2801904, AB_2655772, AB_2655773, AB_2734059

Resources for CD184 (CXCR4) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD184 (CXCR4) Antibody, anti-human, REAfinity™

Publications

  1. McKnight, A. et al. (1997) Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent. J. Virol. 71(2): 1692-1696
  2. Ichiyama, K. et al. (2003) A duodenally absorbable CXC chemokine receptor 4 antagonist, KRH-1636, exhibits a potent and selective anti-HIV-1 activity. Proc. Natl. Acad. Sci. U.S.A. 100: 4185-4190
  3. Berger, E. A. et al. (1999) Chemokine receptors as HIV-1 coreceptors: roles in viral entry, tropism, and disease. Annu. Rev. Immunol. 17: 657-700
  4. Chen, Y. et al. (2015) CXCR4 inhibition in tumor microenvironment facilitates anti-programmed death receptor-1 immunotherapy in sorafenib-treated hepatocellular carcinoma in mice. Hepatology 61(5): 1591-1602
  5. Loetscher, M. et al. (1994) Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. J. Biol. Chem. 269(1): 232-237
  6. Loetscher, P. et al. (2000) Chemokines and their receptors in lymphocyte traffic and HIV infection. Adv. Immunol. 74: 127-180
  7. Chatterjee, S. et al. (2014) The intricate role of CXCR4 in cancer. Adv. Cancer Res. 124: 31-82
  8. McKnight, A. et al. (1997) Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent. J. Virol. 71(2): 1692-1696

Related products for
CD184 (CXCR4) Antibody, anti-human, REAfinity™

3 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?