Clone:
REA1005
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
KIR3DL1, NKB1, NKB1B, p70

Extended validation for CD158e (KIR3DL1) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1005
DX9++
REA970n/a
Cells were incubated with an excess of purified unconjugated CD158e (KIR3DL1) (REA1005) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD158e (KIR3DL1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e (KIR3DL1) antibodies and with a suitable counterstaining. As a control, CD158e (KIR3DL1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158e (KIR3DL1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e (KIR3DL1) antibodies and with a suitable counterstaining. As a control, CD158e (KIR3DL1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158e (KIR3DL1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e (KIR3DL1) antibodies and with a suitable counterstaining. As a control, CD158e (KIR3DL1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158e (KIR3DL1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e (KIR3DL1) antibodies and with a suitable counterstaining. As a control, CD158e (KIR3DL1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158e (KIR3DL1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e (KIR3DL1) antibodies and with a suitable counterstaining. As a control, CD158e (KIR3DL1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158e (KIR3DL1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e (KIR3DL1) antibodies and with a suitable counterstaining. As a control, CD158e (KIR3DL1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158e (KIR3DL1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e (KIR3DL1) antibodies and with a suitable counterstaining. As a control, CD158e (KIR3DL1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD158e (KIR3DL1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e (KIR3DL1) antibodies and with a suitable counterstaining. As a control, CD158e (KIR3DL1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD158e (KIR3DL1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD158e (KIR3DL1) antibodies and with a suitable counterstaining. As a control, CD158e (KIR3DL1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158e (KIR3DL1) (REA1005). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158e (KIR3DL1) (REA1005). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158e (KIR3DL1) (REA1005). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD158e (KIR3DL1) (REA1005). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD158e (KIR3DL1) (REA1005). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD158e (KIR3DL1) Antibody, anti-human, REAfinity™

Overview

Clone REA1005 recognizes the human CD158e (KIR3DL1), a member of the killer immunoglobulin-like receptor (KIR) family expressed on CD56
dim
CD16
+
natural killer (NK) cells and CD8
+
T cells. KIRs contribute to the regulation of NK cell–mediated cytotoxicity. They are monomeric receptors possessing high allelic polymorphism with either 2 or 3 Ig-like extracellular domains. According to the length of their cytoplasmic tail, KIRs can be further subdivided into inhibitory KIRs and activating KIRs (KIR2DS or KIR3DS). KIR3DL1 provides an inhibitory signal of NK cell lytic activity upon interaction with its specific ligand, HLA-Bw4.
Additional information: Clone REA1005 displays negligible binding to Fc receptors.

Alternative names

KIR3DL1, NKB1, NKB1B, p70

Detailed product information

Technical specifications

CloneREA1005
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD158e (KIR3DL1)
Alternative names of antigenKIR3DL1, NKB1, NKB1B, p70
Molecular mass of antigen [kDa]47
Distribution of antigenNK cells, CD8+ T cells
Entrez Gene ID3811
RRIDAB_2727698, AB_2727772, AB_2727699, AB_2727773, AB_2727700, AB_2727774, AB_2727701, AB_2727775, AB_2727702, AB_2727776, AB_2727703, AB_2727777, AB_2727704, AB_2727778, AB_2727705, AB_2727779, AB_2727706, AB_2727780, AB_2727707, AB_2727781, AB_2727708, AB_2727771

Resources for CD158e (KIR3DL1) Antibody, anti-human, REAfinity™

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD158e (KIR3DL1) Antibody, anti-human, REAfinity™

Publications

  1. Selvakumar, A. et al. (1997) Polymorphism and domain variability of human killer cell inhibitory receptors. Immunol. Rev. 155: 183-196
  2. Farag, S. S. et al. (2002) Natural killer cell receptors: new biology and insights into the graft-versus-leukaemia effect. Blood 100(6): 1935-1947
  3. Hsu, K. et al. (2002) The killer cell immunoglobin-like (KIR) genomic region: gene-order, haplotypes and allelic polymorphism. Immunol. Rev. 190: 40-52

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