Clone:
3C11
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC
Alternative names:
c-kit, SCO1, SCO5, SOW3, Ssm, Tr-kit, W, KIT, SCFR

Extended validation for CD117 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 3C11
REA791++
2B8-
ACK2-
180627-
Cells were incubated with an excess of purified unconjugated CD117 (3C11) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD117. Bone marrow cells from C57BL/6 mice were stained with CD117 antibodies and with a suitable counterstaining. As a control, CD117 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD117 (3C11). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD117 (3C11). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD117 (3C11). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD117 Antibody, anti-mouse

Overview

Clone 3C1 recognizes mouse CD117, a 145 kDa cell surface glycoprotein belonging to the class III receptor tyrosine kinase family. CD117 is also known as c-kit or stem cell factor (SCF) receptor. It is expressed on the majority of hematopoietic progenitor cells including multipotent hematopoietic stem cells as well as some committed myeloid, erythroid and lymphoid precursor cells, and mature mast cells. CD117
+
stem cells from murine bone marrow could also be differentiated into smooth muscle cells, myocytes, and endothelial cells
in vivo
.

Alternative names

c-kit, SCO1, SCO5, SOW3, Ssm, Tr-kit, W, KIT, SCFR

Detailed product information

Technical specifications

Clone3C11
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD117
Alternative names of antigenc-kit, SCO1, SCO5, SOW3, Ssm, Tr-kit, W, KIT, SCFR
Molecular mass of antigen [kDa]107
Distribution of antigenB cells, dendritic cells, endothelial cells, fibroblasts, mast cells, megakaryocytes, NK cells, red blood cells, T cells, stem cells, cancer stem cells, hematopoietic stem and progenitor cells, leukemia cells, myeloid dendritic cells, bone marrow, brain, kidney, liver, lung
Entrez Gene ID16590
RRIDAB_2811414, AB_2857758, AB_2660123, AB_2660124, AB_2801976

Resources for CD117 Antibody, anti-mouse

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD117 Antibody, anti-mouse

Publications

  1. Orlic, D. et al. (2001) Mobilized bone marrow cells repair the infarcted heart, improving function and survival. Proc. Natl. Acad. Sci. U.S.A. 98: 10344-10349
  2. Orlic, D. (2002) Stem cell repair in ischemic heart disease: an experimental model. Int. J. Hematol. 76(suppl.1): 144-145

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