Clone:
H4B4
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
ICFC, IF, IHC
Alternative names:
LAMP-2, LAMPB, LGP110

Extended validation for CD107b Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with H4B4
REA1073++
508921+ +
Cells were incubated with an excess of purified unconjugated CD107b (H4B4) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD107b. Jurkat cells were fixed, permeabilized, and then stained with CD107b antibodies and plotted against the side scatter. As a control, CD107b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD107b Antibody, anti-human

Overview

The monoclonal antibody H4B4 reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2), which is a 120 kDa highly glycosylated type I transmembrane protein. It is widely located in lysosomal/endosomal membranes in a high variety of cell types and also expressed on degranulating T cells, activated platelets, and some tumor cell lines. CD107b is involved in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation as well as in tumor cell metastasis.

Alternative names

LAMP-2, LAMPB, LGP110

Detailed product information

Technical specifications

CloneH4B4
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
AntigenCD107b
Alternative names of antigenLAMP-2, LAMPB, LGP110
Molecular mass of antigen [kDa]42
Distribution of antigenleukocytes, platelets, T cells, lymphocytes, tumor cells
Entrez Gene ID3920
RRIDAB_2654501, AB_2654502, AB_2654505, AB_2654506, AB_2654507, AB_2654508, AB_2654509, AB_2654510, AB_2654511, AB_2654512, AB_2654513, AB_2654514

Resources for CD107b Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD107b Antibody, anti-human

Publications

  1. Grutzkau, A. et al. (2004) LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A 61(1): 62-68
  2. Carlsson, S. R. et al. (1988) Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan. J. Biol. Chem. 263: 18911-18919
  3. Betts, M. R. et al. (2004) Detection of T-cell degranulation: CD107a and b. Method Cell Biol. 75: 497-512

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