Clone:
11F2
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1

Specifications for Anti-TCRγ/δ antibodies

Overview

11F2 recognizes the human γ/δ T cell receptor (TCR). The T cell receptor is a heterodimeric glycoprotein associated with the CD3 antigen. The TCR consists of a α and a β chain (TCRα/β) or a γ and a δ chain (TCRγ/δ). Clone 11F2 reacts with a framework epitope of the γ/δ T cell–receptor. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non-disulfide-linked heterodimers with the δ-subunit. The γ/δ T cell–receptor is present on a subset of T lymphocytes in peripheral blood, intestinal epithelium, lymph node, thymus, and spleen. TCR γ/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. γ/δ T cells are mainly CD4 negative and CD8 negative. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. Antigen presentation by γ/δ T cells has been reported.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis. Expression of TCRγ/δ may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Detailed product information

Technical specifications

Clone11F2
Isotypemouse IgG1
Type of antibodyPrimary antibodies
Specieshuman
AntigenTCRγ/δ
Distribution of antigenT cells

Resources for Anti-TCRγ/δ antibodies

Certificates

Please follow this
link
to download the Certificate of Conformity (CoC) by lot number.

References for Anti-TCRγ/δ antibodies

Publications

  1. Brenner, M. B. et al. (1986) Identification of a putative second T-cell receptor. Nature 322(6075): 145-149
  2. Call, M. E. et al. (2002) The organizing principle in the formation of the T cell receptor-CD3 complex. Cell 111(7): 967-979
  3. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
  4. Oettgen, H. C. et al. (1984) Characterization of the two heavy chains of the T3 complex on the surface of human T lymphocytes. J. Biol. Chem. 259(19): 12039-12048
  5. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
  6. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
  7. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
  8. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  9. Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22

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