Background information
Brain tissue from adult mice or rats older than P7 is dissociated into single-cell suspensions. The extracellular matrix is enzymatically digested using the kit components, while the gentleMACS™ Dissociator with Heaters is used for the mechanical dissociation steps during the on-instrument enzyme incubation. After the dissociation, the Debris Removal Solution is used for the elimination of debris, and the Red Blood Cell Removal Solution is used for the removal of erythrocytes.
The single-cell suspension may be analyzed for phenotype distributions, and other functional, genetic, or proteomic studies can be performed. Furthermore, dissociated cells can be cultured, or neurons, astrocytes, or oligodendrocytes can be isolated using MACS
® Technology.
