MBTP13: Immunophenotyping of monocytes from mouse spleen using flow cytometry

Miltenyi Biotec-tested panel 13 (MBTP 13)

This application protocol describes the flow cytometric analysis of monocytes after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.


Gating strategy showing the analysis of monocytes from mouse spleen. Splenocytes from C57BL/6 mice were stained using the previously described panel. Samples were initially gated on single cells using FSC-H/FSC-A gating and on live cells using Viobility-negative gating (data not shown). CD45+ monocytes (A) lack expression of F4/80 (B) as well as T, B, and NK cell lineage markers (CD3, CD19, CD335; C), while expressing high levels of CD11b and Ly6C (C, D). In addition, analysis of the expression of the chemokine receptor CD192 helps to identify monocytes within mouse spleen (D).


Matching products: