Flow cytometry-based quality control assay for PSC-derived midbrain dopaminergic neurons

This application protocol describes an in-process quality control assay to evaluate purity and identity of midbrain dopaminergic neurons differentiated from pluripotent stem cells (PSC-mDA neurons). The step-by-step protocol is based on a flow cytometric approach using the PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™ (# 130-127-439).


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This protocol includes detailed instructions for preparing necessary reagents and materials and then carrying out each step.


The following is a listing of reagents, instruments, and consumables needed for each step of this protocol. These products are for research use only.
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