Clone:
REA473
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MC
Alternative names:
TOX1, KIAA0808

Extended validation for TOX Antibody, anti-human/mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA473
TXRX10++
6E6D03-
Cells were incubated with an excess of purified unconjugated TOX (REA473) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for TOX. Jurkat cells were fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with TOX antibodies and plotted against the side scatter. As a control, TOX antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TOX. Jurkat cells were fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with TOX antibodies and plotted against the side scatter. As a control, TOX antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TOX. Jurkat cells were fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with TOX antibodies and plotted against the side scatter. As a control, TOX antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for TOX. Jurkat cells were fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were then stained with TOX antibodies and plotted against the side scatter. As a control, TOX antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.

Specifications for TOX Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA473 recognizes the human and mouse TOX antigen, a member of an evolutionarily conserved DNA-binding protein family, and also known as thymocyte selection-associated HMG box protein. TOX is a thymic transcript that is highly upregulated in CD4
+
CD8
+
double positive thymocytes. It is expressed in the thymus in a stage-specific regulated manner and in several immune-relevant cell subsets. TOX expression is also detected in other tissues, including liver, where it likely plays additional functions in regulating metabolic processes. It is part of the larger HMG-box superfamily of proteins, but also defines a small subfamily of proteins including TOX2, TOX3, and TOX4, all of which are highly conserved in vertebrate species. While the
in vivo
functions of TOX have been best characterized, TOX2 may play a role in reproductive organs, TOX3 has been implicated in regulation of neuron cell survival and breast cancer, and TOX4 is known to interact with a phosphatase complex involved in the control of chromatin structure and cell cycle progression.
Additional information: Clone REA473 displays negligible binding to Fc receptors.

Alternative names

TOX1, KIAA0808

Detailed product information

Technical specifications

CloneREA473
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenTOX
Alternative names of antigenTOX1, KIAA0808
Molecular mass of antigen [kDa]58, 57(separately shown for human and mouse/rat)
Distribution of antigenT cells, T helper cells, liver
Entrez Gene ID9760
RRIDAB_2751485, AB_2801785, AB_2801780, AB_2751522

Resources for TOX Antibody, anti-human/mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for TOX Antibody, anti-human/mouse, REAfinity™

Publications

  1. Yun, S. et al. (2011)
    TOX regulates the differentiation of human natural killer cells from hematopoietic stem cells
    in vitro
    .
    Immunol. Lett. 136(1): 29-36
  2. Morimura, S. et al. (2014) TOX expression in different subtypes of cutaneous lymphoma. Arch. Dermatol. Res. 306(9): 843-849
  3. Aliahmad, P. et al. (2012) The many roles of TOX in the immune system. Curr. Opin. Immunol. 24(2): 173-177

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