Clone:
GL3
Type of antibody:
Primary antibodies
Isotype:
hamster IgGκ
Applications:
FC, MICS, IF, IHC
Alternative names:
TCRD, Tcrdelta, TCRGV1S1, TCRGV2S1, TCRGV3S1, TCRGV5S3, TCRG

Extended validation for TCRγ/δ Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with GL3
REA633++
UC7-13D5++
Cells were incubated with an excess of purified unconjugated TCRγ/δ (GL3) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (GL3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (GL3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (GL3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCRγ/δ Antibody, anti-mouse

Overview

The T cell receptor is a heterodimeric glycoprotein associated with the CD3 antigen. The TCR consists of a α and a β chain (TCRα/β) or a γ and a δ chain (TCRγ/δ). Clone GL3 reacts with a framework epitope of the γ/δ T-cell receptor. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non-disulfide-linked heterodimers with the δ-subunit. The γ/δ T-cell receptor is present on a subset of T lymphocytes in peripheral blood, intestinal epithelium, lymph node, thymus and spleen. TCR γ/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. γ/δ T cells are mainly CD4 negative and CD8 negative. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. Antigen presentation by γ/δ T cells has been reported.

Alternative names

TCRD, Tcrdelta, TCRGV1S1, TCRGV2S1, TCRGV3S1, TCRGV5S3, TCRG

Detailed product information

Technical specifications

CloneGL3
Clonalitymonoclonal
Isotypehamster IgGκ
Hosthamster
Type of antibodyPrimary antibodies
Speciesmouse
AntigenTCRγ/δ
Alternative names of antigenTCRD, Tcrdelta, TCRGV1S1, TCRGV2S1, TCRGV3S1, TCRGV5S3, TCRG
Distribution of antigenT cells
RRIDAB_2654076, AB_2654077, AB_2654078, AB_2654079, AB_2654080, AB_2654081, AB_2654082, AB_2654083, AB_2654084, AB_2654085, AB_2654086, AB_2654087, AB_2654088, AB_2654089, AB_2654090, AB_2654075

Resources for TCRγ/δ Antibody, anti-mouse

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

Reviews for TCRγ/δ Antibody, anti-mouse

Great Alternative for PE/Cy7

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Anti-TCRγ/δ-PE-Vio770, mouse (130-104-042)

The goal of this experiment was to analyze the frequency of gdT cells among CD3+CD4-CD8- T cells in the lung.

References for TCRγ/δ Antibody, anti-mouse

Publications

  1. Schmolka, N. et al. (2013) Epigenetic and transcriptional signatures of stable versus plastic differentiation of proinflammatory γδ T cell subsets. Nat. Immunol. 14(10): 1093-1100
  2. Prinz, I. et al. (2013) Functional development of γδ T cells. Eur. J. Immunol. 43(8): 1988-1994

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