Clone:
REA662
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC

Extended validation for TCR Vβ1 Antibody, anti-human, REAfinity™

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vβ1 (REA662). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCR Vβ1 (REA662). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCR Vβ1 (REA662). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCR Vβ1 Antibody, anti-human, REAfinity™

Overview

Clone REA662 recognizes the human variable beta 1 subunit of the αβ T cell receptor (TCR Vβ1). The TCR is a heterodimeric glycoprotein associated with the CD3 antigen. The α and β TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. TCR Vβ1 is a variant of the TCR β chain. αβ T cells express a diverse αβ TCR repertoire that specifically co-recognizes self or foreign antigen bound to antigen-presenting molecules, which thereby leads to T cell–mediated immunity. For example, the TCR can directly bind to peptide fragments, riboflavin precursors, and lipid antigens that are presented by major histocompatibility complex (MHC) molecules, MR1 and CD1, respectively. In each case, the antigen sits within the antigen-binding cleft, whereupon the TCR recognizes a composite surface formed by the antigen-presenting molecule and surface-exposed regions of the antigen itself. This co-recognition paradigm is a central tenet of αβ T cell–mediated immunity and underpins MHC restriction.
Additional information: Clone REA662 displays negligible binding to Fc receptors.

Detailed product information

Technical specifications

CloneREA662
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTCR Vβ1
Distribution of antigenT cells
Entrez Gene ID28621 , 6957
RRIDAB_2801781, AB_2653722, AB_2653723, AB_2653724, AB_2653725, AB_2653726, AB_2653727, AB_2653728, AB_2653729, AB_2801786

Resources for TCR Vβ1 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for TCR Vβ1 Antibody, anti-human, REAfinity™

Publications

  1. Born, W. et al. (1985) Rearrangement of T-cell receptor beta-chain genes during T-cell development. Proc. Natl. Acad. Sci. U.S.A. 82(9): 2925-2929
  2. Dunon, D. et al. (1994) T cell migration during development: homing is not related to TCR V beta 1 repertoire selection. EMBO J. 13(4): 808-815
  3. van den Beemd, R. et al. (2000) Flow cytometric analysis of the Vbeta repertoire in healthy controls. Cytometry 40(4): 336-345
  4. Wei, S. et al. (1994) The extent of the human germline T-cell receptor V beta gene segment repertoire. Immunogenetics 40(1): 27-36

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