The StraightFrom
®
Leukopak
®
REAlease
®
MicroBead Kit was developed for the rapid positive isolation of CD56
+
cells directly from Leukopak
®
using the MultiMACS Cell 24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. The Multi-24 Column Block is included in the kit to allow direct processsing of a 1/2 Leukopak
®

Data and images for
StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit
, human

Figures

Figure 1

A) CD56
+
cells were isolated from ½ Leukopak
®
using the StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit, CD3 MicroBeads, Multi-24 Column Block, LD Columns for CD3 depletion, and a MultiMACS™ Cell24 Separator Plus. Cells were fluorescently stained with REAfinity
®
Antibodies CD56-APC, CD3-FITC, and Biotin Antibody-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Biotin Antibody-PE to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin (“MicroBead-free CD56
+
cells”), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
A) Cell purity
Before separation
Label-free CD56
+
cells
Cells after CD3 depletion
View details

Figure 1

A) CD56
+
cells were isolated from ½ Leukopak
®
using the StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit, CD3 MicroBeads, Multi-24 Column Block, LD Columns for CD3 depletion, and a MultiMACS™ Cell24 Separator Plus. Cells were fluorescently stained with REAfinity
®
Antibodies CD56-APC, CD3-FITC, and Biotin Antibody-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Biotin Antibody-PE to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin (“MicroBead-free CD56
+
cells”), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
View details

Figure 1

A) CD56
+
cells were isolated from ½ Leukopak
®
using the StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit, CD3 MicroBeads, Multi-24 Column Block, LD Columns for CD3 depletion, and a MultiMACS™ Cell24 Separator Plus. Cells were fluorescently stained with REAfinity
®
Antibodies CD56-APC, CD3-FITC, and Biotin Antibody-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Biotin Antibody-PE to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin (“MicroBead-free CD56
+
cells”), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
View details

Figure 1

A) CD56
+
cells were isolated from ½ Leukopak
®
using the StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit, CD3 MicroBeads, Multi-24 Column Block, LD Columns for CD3 depletion, and a MultiMACS™ Cell24 Separator Plus. Cells were fluorescently stained with REAfinity
®
Antibodies CD56-APC, CD3-FITC, and Biotin Antibody-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Biotin Antibody-PE to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin (“MicroBead-free CD56
+
cells”), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
B) Label-free cells: REAlease Biotin Complex release
Before separation
MicroBead-free CD56
+
cells
Label-free CD56
+
cells
View details

Figure 1

A) CD56
+
cells were isolated from ½ Leukopak
®
using the StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit, CD3 MicroBeads, Multi-24 Column Block, LD Columns for CD3 depletion, and a MultiMACS™ Cell24 Separator Plus. Cells were fluorescently stained with REAfinity
®
Antibodies CD56-APC, CD3-FITC, and Biotin Antibody-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Biotin Antibody-PE to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin (“MicroBead-free CD56
+
cells”), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
View details

Figure 1

A) CD56
+
cells were isolated from ½ Leukopak
®
using the StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit, CD3 MicroBeads, Multi-24 Column Block, LD Columns for CD3 depletion, and a MultiMACS™ Cell24 Separator Plus. Cells were fluorescently stained with REAfinity
®
Antibodies CD56-APC, CD3-FITC, and Biotin Antibody-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Biotin Antibody-PE to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin (“MicroBead-free CD56
+
cells”), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
View details

Figure 1

A) CD56
+
cells were isolated from ½ Leukopak
®
using the StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit, CD3 MicroBeads, Multi-24 Column Block, LD Columns for CD3 depletion, and a MultiMACS™ Cell24 Separator Plus. Cells were fluorescently stained with REAfinity
®
Antibodies CD56-APC, CD3-FITC, and Biotin Antibody-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Biotin Antibody-PE to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin (“MicroBead-free CD56
+
cells”), whereas the label-free CD56
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.

Figure

View details
Step-by-step separation procedure
Step-by-step separation procedure

Specifications for
StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit
, human

Overview

The StraightFrom
®
Leukopak
®
REAlease
®
MicroBead Kit was developed for the rapid positive isolation of CD56
+
cells directly from Leukopak
®
using the MultiMACS Cell 24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. The Multi-24 Column Block is included in the kit to allow direct processsing of a 1/2 Leukopak
®
.

Detailed product information

Detailed separation procedure

A Leukopak
®
sample is labeled with StraightFrom
®
Leukopak
®
REAlease
®
CD56 Biotin Complex followed by Anti-Biotin MicoBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus. After removing the Multi-24 Column Block from the magnetic field, the magnetically retained CD56
+
cells are eluted as the positively selected cell fraction bead-free, or free of MicroBeads. Optionally, the REAlease Biotin Complex can be removed for label-free target cells.

Applications

CD56
+
cells isolated using StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBeads are ideally suited for further magnetic separation, i.e. for the depletion of CD56
+
CD3
+
cells with CD3 MicroBeads. The cells are suited for further flow cytometric analyses, molecular biology applications and functional studies.

Columns

Multi-24 Column Block

Resources for
StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit
, human

Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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StraightFrom
®
Leukopak
®
REAlease
®
CD56 MicroBead Kit
, human

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