Clone:
REA278
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MC
Alternative names:
RORC, NR1F3, RORG, RZR-GAMMA, RZRG, TOR, RORgamma, Thor

Extended validation for RORγ (t) Antibody, anti-human/mouse, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for RORγ (t). Thymocytes derived from B57BL/6 mice were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with RORγ (t) antibodies. As a control, RORγ (t) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for RORγ (t). Thymocytes derived from B57BL/6 mice were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with RORγ (t) antibodies. As a control, RORγ (t) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for RORγ (t). Thymocytes derived from B57BL/6 mice were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with RORγ (t) antibodies. As a control, RORγ (t) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for RORγ (t). Thymocytes derived from B57BL/6 mice were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with RORγ (t) antibodies. As a control, RORγ (t) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for RORγ (t). Thymocytes derived from B57BL/6 mice were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with RORγ (t) antibodies. As a control, RORγ (t) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for RORγ (t) Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA278 recognizes the retinoid-related orphan receptor-γ (RORγ) antigen and the RORγ (t) isoform, which are ligand-dependent transcription factors that play roles in multiple physiological processes. RORγ is expressed with highest levels in several tissues like muscle, kidney, and liver, whereas RORγ (t) is expressed almost exclusively in immature CD4
+
CD8
+
double-positive thymocytes. RORγ is the possible nuclear receptor for hydroxycholesterols, the binding of which strongly promotes coactivator recruitment. It is essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes. RORγ (t) negatively regulates expression of Fas ligand and IL-2 production in T cells. It also binds to the TEA promoter and may be part of the pre-TCR activation cascade leading to the maturation of α/β T cells and may participate in the regulation of DNA accessibility in the TCR-Jα locus.
Additional information: Clone REA278 displays negligible binding to Fc receptors.

Alternative names

RORC, NR1F3, RORG, RZR-GAMMA, RZRG, TOR, RORgamma, Thor

Detailed product information

Technical specifications

CloneREA278
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenRORγ (t)
Alternative names of antigenRORC, NR1F3, RORG, RZR-GAMMA, RZRG, TOR, RORgamma, Thor
Molecular mass of antigen [kDa]58 (56)(antibody detects two isoforms, molecular weight in brackets is for RORγ (t))
Distribution of antigenlymphocytes, thymocytes
Entrez Gene ID6097
RRIDAB_2802014, AB_2811585, AB_2811561, AB_2653369

Resources for RORγ (t) Antibody, anti-human/mouse, REAfinity™

Certificates

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References for RORγ (t) Antibody, anti-human/mouse, REAfinity™

Publications

  1. He, Y. W. et al. (1998) RORγ t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells. Immunity 9(6): 797-806
  2. Villey, I. et al. (1999) RORγT, a thymus-specific isoform of the orphan nuclear receptor RORγ / TOR, is up-regulated by signaling through the pre-T cell receptor and binds to the TEA promoter. Eur. J. Immunol. 29(12): 4072-4080
  3. Jin, L. et al. (2010) Structural basis for hydroxycholesterols as natural ligands of orphan nuclear receptor RORγ. Mol Endocrinol. 24(5): 923-929
  4. Moretti, S. et al. (2017) A mast cell-ILC2-Tʜ9 pathway promotes lung inflammation in cystic fibrosis. Nat Commun. 8: 14017

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