These kits and tool boxes have been developed for the isolation of rat retinal ganglion cells (RGCs).

Data and images for Retinal Ganglion Cell Isolation Kits, rat

Figures

Figure 1

Isolation of CD90.1
+
RGCs from postnatal day 7 rat retinal tissue using the Neural Tissue Dissociation Kit - Postnatal Neurons, the Retinal Ganglion Cell Isolation Kit, a MACSiMAG Separator, an OctoMACS™ Separator, and MS Columns. Cells were fluorescently stained with CD48-PE and CD90.1-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
CD90.1
+
retinal ganglion cells
View details

Figure 1

Isolation of CD90.1
+
RGCs from postnatal day 7 rat retinal tissue using the Neural Tissue Dissociation Kit - Postnatal Neurons, the Retinal Ganglion Cell Isolation Kit, a MACSiMAG Separator, an OctoMACS™ Separator, and MS Columns. Cells were fluorescently stained with CD48-PE and CD90.1-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Isolation of CD90.1
+
RGCs from postnatal day 7 rat retinal tissue using the Neural Tissue Dissociation Kit - Postnatal Neurons, the Retinal Ganglion Cell Isolation Kit, a MACSiMAG Separator, an OctoMACS™ Separator, and MS Columns. Cells were fluorescently stained with CD48-PE and CD90.1-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Retinal Ganglion Cell Isolation Kits, rat

Overview

These kits and tool boxes have been developed for the isolation of rat retinal ganglion cells (RGCs).

Detailed product information

Background information

Retinal ganglion cells (RGCs) are neurons of the central nervous system, which transmit information from the retina to the visual center of the brain. RGCs have been used to study neuron-glia interactions and the mechanisms of neurodegeneration.
1–4

Detailed separation procedure

The separation procedure involves the depletion of endothelial cells and microglia using MACSiBead™ Particles, and the positive selection of CD90.1
+
RGCs using CD90.1 MicroBeads.
5
First, the cell suspension is incubated with a biotin-conjugated antibody, which binds to endothelial cells and microglia, and with CD90.1 MicroBeads labeling RGCs. In the next step, endothelial cells and microglia are magnetically labeled with Anti-Biotin MACSiBead Particles and depleted by using a MACSiMAG™ Separator. Subsequently, the cell fraction depleted of endothelial cells and microglia is applied to MS Columns for positive selection of the CD90.1
+
RGCs.
Note: MACSiBead Particles are
not
suitable for separations using MACS Columns and MiniMACS™, MidiMACS™, VarioMACS™, SuperMACS™, or autoMACS® Separators.

Columns

For positive selection: MS Columns.

Resources for Retinal Ganglion Cell Isolation Kits, rat

References for Retinal Ganglion Cell Isolation Kits, rat

Publications

  1. Barres, B. A. et al. (1988) Immunological, morphological, and electrophysiological variation among retinal ganglion cells purified by panning. Neuron 1: 791-803
  2. Meyer-Franke, A. et al. (1995) Characterization of the signaling interactions that promote the survival and growth of developing retinal ganglion cells in culture. Neuron 15: 805-819
  3. Pfrieger, F. W. and Barres, B. A. (1997)
    Synaptic efficacy enhanced by glial cells
    in vitro
    .
    Science 277: 1684-1687
  4. Göritz et al. (2007) Glia-induced neuronal differentiation by transcriptional regulation. Glia 55: 1108-1122
  5. Pennartz et al. (2010) MACS&more 12(2): 16-18

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