Clone:
REA523
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
H2-Qa2, H2-Q7

Extended validation for QA-2 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA523
69H1-9-9++
1-1-2-
Cells were incubated with an excess of purified unconjugated QA-2 (REA523) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones forQA-2. C57BL6/6 splenocytes were stained with QA-2 antibodies and with a suitable counterstaining. As a control, QA-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones forQA-2. C57BL6/6 splenocytes were stained with QA-2 antibodies and with a suitable counterstaining. As a control, QA-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones forQA-2. C57BL6/6 splenocytes were stained with QA-2 antibodies and with a suitable counterstaining. As a control, QA-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones forQA-2. C57BL6/6 splenocytes were stained with QA-2 antibodies and with a suitable counterstaining. As a control, QA-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using QA-2 (REA523). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using QA-2 (REA523). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using QA-2 (REA523). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for QA-2 Antibody, anti-mouse, REAfinity™

Overview

Clone REA523 recognizes the mouse Qa-2 antigen, a 40 kDa single-pass type I membrane protein, which is also known as H-2 class I histocompatibility antigen Q7 alpha chain (H2-Q7). Qa-2 is a murine non-classical MHC class I molecule involved in the modulation of immune responses by interacting with T CD8
+
and NK cell inhibitory receptors. The murine Qa-2 genes map to the H-2Q region, between the classical class I H-2D locus and the H-2TL cluster of class lb sequences. This locus encodes a variable number of class Ib genes in different strains. Strains expressing high (Qa-2
high
), medium (Qa-2
med
), low (Qa-2
low
), and no Qa-2 (Qa-2
nul
) were identified. Qa-2 is found as membrane-bound and soluble isoforms generated by alternative splicing. It is expressed mostly by lymphoid-derived cells and plays an important role in controlling growth and murine fetal development and, like HLA-G, the molecule is related to the protection of the fetus by inhibiting maternal NK cell-mediated lysis.
Additional information: Clone REA523 displays negligible binding to Fc receptors.

Alternative names

H2-Qa2, H2-Q7

Detailed product information

Technical specifications

CloneREA523
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenQA-2
Alternative names of antigenH2-Qa2, H2-Q7
Molecular mass of antigen [kDa]36(average of subunits)
Distribution of antigenB cells, T cells, NK cells, T helper cells, NKT cells
Entrez Gene ID15018, 110558, 15019
RRIDAB_2653307, AB_2653308, AB_2653309, AB_2653310, AB_2653311, AB_2653312, AB_2653313, AB_2653314, AB_2653315, AB_2653316, AB_2653317, AB_2653318, AB_2653319, AB_2653306

Resources for QA-2 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for QA-2 Antibody, anti-mouse, REAfinity™

Publications

  1. Melo-Lima, B. L. et al. (2014) Differential transcript profiles of MHC class Ib(Qa-1, Qa-2, and Qa-10) and Aire genes during the ontogeny of thymus and other tissues. J. Immunol.
  2. Devlin, J. J. et al. (1985) Duplicated gene pairs and alleles of class I genes in the Qa2 region of the murine major histocompatibility complex: a comparison. EMBO J. 4(12): 3203-3207
  3. Lu, N. et al. (2011) Dynamic expression of Qa-2 during acute graft rejection. Mol. Med. 17(3-4): 248-255

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