Clone:
REA704
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
NR4A1, TR3, NGFI-B, NAK1, N10, TISI

Extended validation for Nur77 Antibody, anti-mouse, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Nur77. Thymocytes from C57BL/6 were stimulated with 20ng/ml Phorbol-12-myristat-13-acetat (PMA) and 500ng/ml Ionomycin for 2 hours. Stimulated cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Nur77 antibodies. As a control, Nur77 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Nur77. Thymocytes from C57BL/6 were stimulated with 20ng/ml Phorbol-12-myristat-13-acetat (PMA) and 500ng/ml Ionomycin for 2 hours. Stimulated cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Nur77 antibodies. As a control, Nur77 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Nur77. Thymocytes from C57BL/6 were stimulated with 20ng/ml Phorbol-12-myristat-13-acetat (PMA) and 500ng/ml Ionomycin for 2 hours. Stimulated cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Nur77 antibodies. As a control, Nur77 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Nur77. Thymocytes from C57BL/6 were stimulated with 20ng/ml Phorbol-12-myristat-13-acetat (PMA) and 500ng/ml Ionomycin for 2 hours. Stimulated cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Nur77 antibodies. As a control, Nur77 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Nur77 Antibody, anti-mouse, REAfinity™

Overview

Clone REA704 recognizes the mouse Nur77 antigen, a member of the steroid/ thyroid hormone receptor superfamily of intracellular transcription factors. Nur77 is an inducible orphan nuclear receptor also known as NR4A1 or nerve growth factor IB (NGFI-B) and plays a role in cell cycle mediation, inflammation, and apoptosis and is involved in mediation of inflammatory responses in macrophages. Nur77 expression is found on thymocytes and T cell lines and is mainly expressed in testis but also in brain and muscle tissue.
Additional information: Clone REA704 displays negligible binding to Fc receptors.

Alternative names

NR4A1, TR3, NGFI-B, NAK1, N10, TISI

Detailed product information

Technical specifications

CloneREA704
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse, human
Cross-reactivityhuman
AntigenNur77
Alternative names of antigenNR4A1, TR3, NGFI-B, NAK1, N10, TISI
Molecular mass of antigen [kDa]65
Distribution of antigenT cells, thymocytes, brain, muscle, testicle
Entrez Gene ID15370
RRIDAB_2653069, AB_2653070, AB_2653071, AB_2653068

Resources for Nur77 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Nur77 Antibody, anti-mouse, REAfinity™

Publications

  1. Cunningham, N. R. et al. (2006)
    Immature CD4
    +
    CD8
    +
    thymocytes and mature T cells regulate Nur77 distinctly in response to TCR stimulation.
    J. Immunol. 177(10): 6660-6666
  2. Pei, L. et al. (2006) Regulation of macrophage inflammatory gene expression by the orphan nuclear receptor Nur77. Mol Endocrinol. 20(4): 786-794
  3. Tao, R. et al. (2008)
    Resistance of Foxp3
    +
    regulatory T cells to Nur77-induced apoptosis promotes allograft survival.
    PLoS One 3(5): e2321

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